Sökning: "model library design"

Visar resultat 21 - 25 av 56 avhandlingar innehållade orden model library design.

  1. 21. Expression of a lipase in prokaryote and eukaryote host systems allowing engineering

    Författare :Marianne Wittrup Larsen; Karl Hult; Marina Lotti; KTH; []
    Nyckelord :pseudozzyma candida antarctica lipase B; escherichia coli; pichia pastoris; rational design;

    Sammanfattning : Pseudozyma (Candida) antarctica lipase B (PalB) was expressed in Escherichia coli facilitating protein engineering. The lack of glycosylation was evaluated for a deeper understanding of the difficulties in expressing PalB in E. coli. LÄS MER

  2. 22. Industrial Bioprocess Developments for Biogas and Ethanol Production

    Författare :Karthik Rajendran; Högskolan i Borås; []
    Nyckelord :ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; biogas; ethanol; process design; techno-economic analysis; simulation; modeling; Resource Recovery; Resursåtervinning;

    Sammanfattning : Current biofuels face a noteworthy misfortune on commercialization because of its economiccomparison with low-cost fuel from the oil reserves. To compete with gasoline as a fuel, thebiofuels need to be economically feasible and demonstrated on a large-scale. LÄS MER

  3. 23. Large Eddy Simulation of Turbulent Swirling Flows and Turbulent Premixed Combustion

    Författare :Ping Wang; Strömningsteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; fluid dynamics; Gases; flamelet model; flame wrinkle; large eddy simulation; vortex breakdown; turbulent premixed combustion; level set G-equation; swirling flow; plasmas; Gaser; fluiddynamik; plasma; Energy research; Energiforskning;

    Sammanfattning : With the increasing concerns about our environment and human health, more and more stringent emission limits have been specified. These adopted and still coming emission limits require and promote more accurate, less experiential methods to design and develop the combustors. LÄS MER

  4. 24. Design of Glutathione Transferase Variants for Novel Activities with Alternative Substrates

    Författare :Abeer Shokeer; Bengt Mannervik; Francoise Raffalli-Mathieu; David Josephy; Uppsala universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; NATURAL SCIENCES; Biochemistry; Biokemi; Organic chemistry; Organisk kemi; Biokemi; Biochemistry; Organisk kemi; Organic Chemistry;

    Sammanfattning : Glutathione transferases (GSTs) play a pivotal role in cellular defense, since they are main contributors to the inactivation of genotoxic compounds of exogenous and endogenous origins. Directed evolution was used to improve the catalytic activities of Theta class GST T1-1 toward different substrates. LÄS MER

  5. 25. Gene fusion in protein engineering : design of novel peptides and bifunctional enzymes

    Författare :Sarah Fredriksson; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Cadmium; Bifunctional enzyme; Catalytic triad; Channeling; Coenzyme recycling; Galactose dehydrogenase; ß-Galactosidase; Gene fusion; Hexapeptide; Lactate dehydrogenase; Linker region; Multifunctional enzymes; Oligomeric protein; NADH; Phage; OmpA; Biochemistry; Biokemi; metabolism; Biotechnology; Bioteknik;

    Sammanfattning : The gene fusion technique is widely used in protein engineering and in this thesis it constitutes the basis of both the construction of artificial bifunctional enzymes and the display of two novel peptides. Artificial bifunctional enzymes have been used as model systems in the studies of native multifunctional enzymes and proximity effects within these complexes. LÄS MER