Sökning: "microfluidic enrichment"

Visar resultat 1 - 5 av 18 avhandlingar innehållade orden microfluidic enrichment.

1. 1. Single Molecule Detection : Microfluidic Automation and Digital Quantification

   Författare :Malte Kühnemund; Mats Nilsson; Ulf Landegren; Sten Linnarsson; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; single molecule; digital; rolling circle amplification; magnetic particle; padlock probe; microfluidics; resistive pulse sensing; lab on chip; mobile phone microscopy; enrichment; sequencing;

   Sammanfattning : Much of recent progress in medical research and diagnostics has been enabled through the advances in molecular analysis technologies, which now permit the detection and analysis of single molecules with high sensitivity and specificity. Assay sensitivity is fundamentally limited by the efficiency of the detection method used for read-out. LÄS MER

3. 2. Microfluidics in Surface Modified PDMS : Towards Miniaturized Diagnostic Tools

   Författare :Sara Thorslund; Fredrik Nikolajeff; Jonas Bergquist; Elisabeth Verpoorte; Uppsala universitet; []
   Nyckelord :Materials science; µTAS; micro total analysis system; PDMS; poly dimethylsiloxane ; microfluidics; heparin; blood filtration; on-chip; ESI-MS; desalting; QCM-D; biocompatible; CD4; capillary flow; lab-on-chip; microfabrication; enrichment; point-of-care; hydrophilic; oxidation; Materialvetenskap;

   Sammanfattning : There is a strong trend in fabricating miniaturized total analytical systems, µTAS, for various biochemical and cell biology applications. These miniaturized systems could e.g. gain better separation performances, be faster, consume less expensive reagents and be used for studies that are difficult to access in the macro world. LÄS MER

4. 3. Acoustic manipulation of cells and microbeads in droplet microfluidics

   Författare :Anna Fornell; Avdelningen för Biomedicinsk teknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Acoustophoresis; Droplet microfluidics; Enrichment; Lab-on-a-chip; Particle manipulation; Ultrasound;

   Sammanfattning : Droplet microfluidics has emerged as a promising platform for miniaturisation of biological assays on-chip. In droplet microfluidics small water droplets (nL-pL) surrounded by an immiscible carrier oil are generated at high throughput. LÄS MER

5. 4. Advancing isothermal nucleic acid amplification tests : Towards democratization of diagnostics

   Författare :Felix Neumann; Mats Nilsson; Paul Yager; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; molecular diagnostics; infectious diseases; point-of-care; digital quantification; fluorescence detection; rolling circle amplification; padlock probes; microfluidic enrichment; Biochemistry; biokemi;

   Sammanfattning : Modern healthcare is the result of scientific advancement across disciplines and has enabled us to understand the rationale behind many diseases and how to treat or cure them; but still a myriad of unanswered questions remains. Especially infectious diseases play an important role in healthcare as they pose a constant threat for global health and well-being. LÄS MER

7. 5. Microfluidic and Molecular Tools for Genetic Analyses

   Författare :Henrik Johansson; Mats Nilsson; Ivo Gut; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; microfluidics; PCR; sequencing; cancer genetics; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Medical Genetics; Medicinsk genetik;

   Sammanfattning : Methods that enable interrogation of multiple genomic regions in parallel are very useful for efficient detection of genetic variation. Two different types of probes are described in this thesis that can be used for direct analysis or for sample preparation upstream of Next Generation Sequencing. LÄS MER