Sökning: "microcell hybrids"

Hittade 5 avhandlingar innehållade orden microcell hybrids.

  1. 1. Identification of the common eliminated region (CER1) by the microcell hybrid based 'elimination test'

    Författare :Ying Yang; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Elimination test; common eliminated region 1 CER1 ; 3p21.3; microcell hybrids; tumor suppressor gene; LF lactoferrin ; LIMD1 LIM-domain containing gene 1 ; sequencing; MMCT microcell mediated chromosome transfer ; real time PM methylation;

    Sammanfattning : The aim of elimination test (Et) is to identify tumor antagonizing genes from chromosome regions that are regularly deleted in microcell hybrid (MCH) derived tumors through serial SCID mice passages. Et so far was focused on the short arm of human chromosome 3 (chr 3) due to its frequent deletions in 21 tumor types including renal cell carcinoma (RCC), small cell lung carcinoma (SCLC) and breast cancer (BRC). LÄS MER

  3. 2. A microcell hybrid based 'elimination test' in the search for malignancy related genes

    Författare :Stephan Imreh; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : This thesis is focused on the elimination test developed by us (Imreh et al, 1994). The elimination test was designed as a functional test for malignancy related gene identification. Its task is to follow the cytogenetic and molecular alterations on single normal chromosomes transferred into tumor cells. LÄS MER

  4. 3. A microcell hybrid based elimination test to identify human chromosome 3 regions that antagonize tumor growth

    Författare :Irina Kholodnyuk Holodnuka; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Deletions at multiple sites on the short arm of chr3 have been detected in many different types of human tumors. Each of the deleted regions is expected to harbor a tumor antagonizing/suppressor gene(s) (TSG). The earlier studies showed that the tumorigenicity of malignant cells could be suppressed by introducing a single human chr3. LÄS MER

  5. 4. Characterization of a putative tumor suppressor region identified by the elimination test on human 3p21.3

    Författare :Hajnalka Kiss; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Elimination test; common eliminated region 1 CER1 ; 3p21.3; tumor suppressor gene; large scale sequencing; LIMD1; LZTFL1; FYC01; TMEM7; LRRC2; LUZP3.;

    Sammanfattning : An experimental system, called the elimination test (Et), was developed to identify tumor growth antagonizing genes. Chromosome 3 containing microcell hybrids (MCHs) were passaged through SCID mice and regular deletion of the short arm of chromosome 3 (3p) were found by cytogenetic and molecular methods. LÄS MER

  7. 5. Involvement of evolutionarily plastic regions in cancer associated CHR3 aberrations

    Författare :Eva Darai-Ramqvist; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : A functional test to identify tumor antagonizing regions on chromosome 3 (chr3), called the Elimination Test, was developed in our group. It is based on microcell mediated transfer of human chr3 into mouse or human tumor cells and analysis of the monochromosomal hybrids after their growth in vivo. LÄS MER