Sökning: "membrane protein over-expression"

Visar resultat 1 - 5 av 13 avhandlingar innehållade orden membrane protein over-expression.

1. 1. Into the Membrane and Beyond : Improving Membrane Protein Over-Expression in Escherichia coli

   Författare :Mirjam Klepsch; Jan-Willem de Gier; Reinhard Grisshammer; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; membrane protein over-expression; proteomics; T7 RNA polymerase; peptide transport; strain engineering; Biochemistry; Biokemi; Biophysical chemistry; Biofysikalisk kemi; biokemi; Biochemistry;

   Sammanfattning : Membrane proteins fulfil a wide variety of essential functions in the cell and many are (potential) drug targets. Since their natural abundance is usually very low, most membrane proteins have to be over-expressed for functional and structural studies. LÄS MER

3. 2. Engineering membrane proteins for production and topology

   Författare :Stephen Toddo; Daniel Daley; Roslyn Bill; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Membrane protein; Over-expression; Protein production; Codon optimization; Escherichia coli; AraH; NarK; mRNA secondary structure; coding sequence; ribosome binding site; RBS; topology; split-GFP; Biochemistry; biokemi;

   Sammanfattning : The genomes of diverse organisms are predicted to contain 20 – 30% membrane protein encoding genes and more than half of all therapeutics target membrane proteins. However, only 2% of crystal structures deposited in the protein data bank represent integral membrane proteins. LÄS MER

4. 3. Assay development for studies of G protein-coupled receptors at the single-molecule level

   Författare :Olov Wahlsten; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TIRF microscopy; colocalization; drug discovery; QCM-D; GPCR; pharmacodynamics; kinetics; equilibrium fluctuation analysis;

   Sammanfattning : G protein-coupled receptors (GPCRs), also known as seven transmembrane (7TM) receptors,is the largest family of cell surface receptors. These receptors play a key role in transmittinga wide variety of signals across the cell membrane and are involved in physiologicalprocesses such as sensory transduction, cell-cell communication, neuronal transmission,and hormonal signaling. LÄS MER

5. 4. Sorting nexin 9 in clathrin-mediated endocytosis

   Författare :Richard Lundmark; Sven Carlsson; Elizabeth Smythe; Umeå universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Cell and molecular biology; sorting nexin; dynamin; clathrin; endocytosis; human; adaptor protein complexes; Cell- och molekylärbiologi; Cell and molecular biology; Cell- och molekylärbiologi; medicinsk biokemi; Medical Biochemistry;

   Sammanfattning : Clathrin-mediated endocytosis is a process by which cells can internalise diverse molecules such as nutrients, antigens and signalling-surface receptors. The creation of clathrin-coated vesicles demands interplay between the plasma membrane lipids, cargo molecules and the proteins that build up the coat. LÄS MER

7. 5. The functional organization of nuclear envelope proteins

   Författare :Santhosh Kumar Gudise; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : In eukaryotic cells, the nucleus is enclosed by a double lipid membrane, termed the nuclear envelope (NE). The NE consists of the outer nuclear membrane (ONM), the inner nuclear membrane (INM), the nuclear pore complexes (NPCs) and the nuclear lamina. LÄS MER