Sökning: "mRNA stability"

Visar resultat 21 - 25 av 118 avhandlingar innehållade orden mRNA stability.

1. 21. Nucleic Acids as Drugs and Drug Targets With Focus on Anticancer Active Platinum Complexes and Small Interfering RNAs

   Författare :Hanna Hedman; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Anticancer drugs; platinum complexes; cisplatin; oxaliplatin; nucleic acid; RNA; DNA; siRNA; thermodynamic stability; mass spectrometry; MALDI-MS; chemical probing; luciferase assay;

   Sammanfattning : To ensure that patients receive a safe and effective treatment, it is important to understand how drugs interact with and affect molecules within a cell. Here, evaluation of platinum based drugs and their ability to interfere with nucleic acid functions is presented. LÄS MER

3. 22. Targeting Infectious Disease : Structural and functional studies of proteins from two RNA viruses and Mycobacterium tuberculosis

   Författare :Anna M. Jansson; T. Alwyn Jones; Sherry L. Mowbray; E. Yvonne Jones; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; RNA virus; coronavirus; alphacoronavirus; nsp1; TGEV; flavivirus; Modoc virus; NS5; methyltransferase; mRNA capping; Mycobacterium tuberculosis; tuberculosis; 1-deoxy-D-xylulose 5-phosphate reductoisomerase; DXR; fosmidomycin analogues; MEP pathway; drug development; xray-crystallography; Biology with specialization in Structural Biology; Biologi med inriktning mot strukturbiologi; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi; Biokemi; Biochemistry; Medicinal Chemistry; Läkemedelskemi;

   Sammanfattning : The recent emergence of a number of new viral diseases as well as the re-emergence of tuberculosis (TB), indicate an urgent need for new drugs against viral and bacterial infections.Coronavirus nsp1 has been shown to induce suppression of host gene expression and interfere with host immune response. LÄS MER

4. 23. Nucleic Acids as Drug Targets -Interactions of Platinum Complexes, Polyamines, and siRNA with DNA and RNA Models

   Författare :Margareta Hägerlöf; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Kinetics; Kemi; Chemistry; AU-rich region; siRNA; Circular Dichroism; Polyamine; Modified Nucleoside; HPLC; Thermodynamic Stability; cisplatin; Hairpin; DNA and RNA conformation; RNA; DNA; Anticancer Drug; Platinum Complexes;

   Sammanfattning : Drugs targeting nucleic acids were studied in DNA and RNA model systems. The effects were investigated with respect to structural and thermodynamic influence, kinetics, and biological function. The drugs employed in this thesis were anticancer active platinum(II) complexes, polyamine derivatives, and siRNA. LÄS MER

5. 24. Conformationally Constrained Oligonucleotides for RNA Targeting

   Författare :Qing Li; Jyoti Chattopadhyaya; Li-He Zhang; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; conformationally constrained nucleoside; antisense oligonucleotide; RNA affinity; nuclease stability; RNase H; RNA degradation; Chemistry with specialization in Bioorganic Chemistry; Kemi med inriktning mot bioorganisk kemi;

   Sammanfattning : A short oligonucleotide sequence as in a single-stranded antisense oligo nucleotides (AON) or in double-stranded small interfering RNAs (siRNA) can modulate the gene expression by targeting against the cellular mRNA, which can be potentially exploited for therapeutic purposes in the treatment of different diseases. In order to improve the efficacy of oligonucleotide-based drugs, the problem of target affinity, nuclease stability and delivery needs to be addressed. LÄS MER

7. 25. The Role of RNA Binding Proteins in Insulin Messenger Stability and Translation

   Författare :Rikard G. Fred; Nils Welsh; Michele Solimena; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Medical cell biology; Medicinsk cellbiologi; Medical Cell Biology; Medicinsk cellbiologi;

   Sammanfattning : Although the reason for insufficient release of insulin in diabetes mellitus may vary depending on the type and stage of the disease, it is of vital importance that an amplified insulin biosynthesis can meet the increased need during periods of hyperglycemia. The insulin mRNA is highly abundant in beta cells and changes in insulin mRNA levels are, at least in part, controlled by altered rates of mRNA degradation. LÄS MER