Sökning: "mRNA analyses"

Visar resultat 1 - 5 av 135 avhandlingar innehållade orden mRNA analyses.

  1. 1. Expression and Mutation Analyses of Candidate Cancer Genes In Situ

    Författare :Sara Kiflemariam; Tobias Sjöblom; Fredrik Pontén; Mef Nilbert; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; cancer; in situ; in situ hybridization; padlock probes; mRNA; tyrosine kinases; tyrosine phosphatases; fusion transcripts;

    Sammanfattning : Cancers display heterogeneity in genetic profiles of the individual cancer cells and in the composition of different malignant and non-malignant cell populations. Such intra-tumor heterogeneity plays a role in treatment response and the emergence of resistance to cancer therapies. LÄS MER

  3. 2. Expression profiling using manifold supports

    Författare :Anette Hagberg; Joakim Lundeberg; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; mRNA; expression; minimal residual disease MRD ; chronic myeloid leukemia CML ; recombinant human erythropoietin r-HuEpo ; anemia; insulin dependent diabetes mellitus IDDM ; transplantation; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

    Sammanfattning : Analyses of mRNA provides a condensed view of gene structure and quantitative analysis can reveal the induction of physiological or pathological gene expression programs. This thesis describes a new method for mRNA isolation, followed by sensitive real time detection via polymerase chain reaction (PCR), in order to quantitate transcripts of interest. LÄS MER

  4. 3. From behaviour to genes: anti-predator responses of brown trout (Salmo trutta) under winter conditions

    Författare :Karl Filipsson; Eva Bergman; Ann Erlandsson; Martin Österling; Johan Watz; Stefán Óli Steingrímsson; Karlstads universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; burbot; climate change; cortisol; diel behaviour; gene expression; HPI axis; light; mRNA; piscivorous; poikilotherm; predator; salmonid; stream; stress; temperature; thermal; winter behaviour; Biology; Biologi;

    Sammanfattning : Temperature has major effects on the performance of poikilotherms. In encounters with predators, low winter temperatures constrain predator detection and escape capabilities in prey fishes. LÄS MER

  5. 4. Development of novel molecular methods for Campylobacter species and for Helicobacter pylori

    Författare :Kristina Blom; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Campylobacter; Helicobacter pylori; PCR; mRNA; hybridization; flow cytometry; HpaA; urease; NAP;

    Sammanfattning : Background and Aim: Different Campylobacter species and Helicobacter pylori are common causes of gastrointestinal infections that may result in diarrhea and chronic gastritis, respectively. The overall aim of this thesis was to develop novel methods for identification, differentiation and characterization of these bacteria. LÄS MER

  7. 5. Analyses of genomic and gene expression signatures

    Författare :Rickard Sandberg; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Microarray; comparative genomics; differential gene expression; cell lines; computational biology;

    Sammanfattning : Biology has entered a challenging, information-intense period where computational experiments are complementing traditional experiments. A plethora of new techniques have allowed biological processes to be investigated on a global scale. LÄS MER