Sökning: "lysine 216"

Hittade 3 avhandlingar innehållade orden lysine 216.

  1. 1. A Novel Route for Construction of Multipurpose Receptors through Chemical Modification of Glutathione Transferases

    Författare :Johan Viljanen; Bengt Harald Jonsson; Kerstin Broo; Sophia Hober; Linköpings universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Human GST A1-1; site-specific covalent modification; tyrosine 9; lysine 216; methionine 208; multipurpose receptor; pattern recognition; protein purification; Chemistry; Kemi;

    Sammanfattning : This thesis describes how the human Alpha class glutathione transferase (GST) A1-1 can be reprogrammed either to function as a multipurpose biosensor for detection of small molecule analytes, or as a handle providing for more efficient protein purification.A novel, user-friendly, and efficient method for site-specific introduction of functional groups into the active site of hGST A1-1 is the platform for these achievements. LÄS MER

  3. 2. Multilayer Structures for Biomaterial Applications : Biomacromolecule-based Coatings

    Författare :Tobias Halthur; Per M. Claesson; Bjørn Stokke; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; multilayer; layer-by-layer deposition; physical chemistry; surface chemistry; adsorption; ellipsometry; QCM-D; DPI; protein adsorption; polypeptides; biomaterials; biosurfaces; amelogenin; solid liquid interface; Surface and colloid chemistry; Yt- och kolloidkemi;

    Sammanfattning : The cellular response to a biomaterial, such as a dental implant, is mainly governed by the surface properties, and can thus be altered by the introduction of a surface coating. In this thesis the buildup of a biomacromolecule-based coating formed by layerby-layer (LbL) deposition of the charged polypeptides poly(L-lysine) (PLL) and poly(L-glutamic acid) (PGA) has been studied. LÄS MER

  4. 3. Catalysis and Site-Specific Modification of Glutathione Transferases Enabled by Rational Design

    Författare :Sofia Håkansson Hederos; Lars Baltzer; Kerstin Broo; Linköpings universitet; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Catalysis; Glutathione Transferases; Rational design; Protein design; Site-specific modification; thiolester hydrolysis; Enzyme engineering; Enzymteknik;

    Sammanfattning : This thesis describes the rational design of a novel enzyme, a thiolester hydrolase, derived from human glutathione transferase (GST) A1-1 by the introduction of a single histidine residue. The first section of the thesis describes the design and the determination of the reaction mechanism. LÄS MER