Sökning: "linear dichroism LD"

Visar resultat 1 - 5 av 10 avhandlingar innehållade orden linear dichroism LD.

  1. 1. Biophysical studies of membrane interacting peptides derived from viral and Prion proteins

    Författare :Kamila Oglęcka; Astrid Gräslund; Stephen White; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Prion peptides; Doppel peptide; Influenza fusion peptides; peptide-membrane interactions; translocation; linear dichroism; circular dichroism; Biophysics; Biofysik; Biophysics; biofysik;

    Sammanfattning : This thesis focuses on peptides derived from the Prion, Doppel and Influenza haemagglutinin proteins in the context of bilayer interactions with model membranes and live cells. The studies involve spectroscopic techniques like fluorescence, fluorescence correlation spectroscopy (FCS), circular and linear dichroism (CD and LD), confocal fluorescence microscopy and NMR. LÄS MER

  3. 2. Excitation and Excited State Properties of Heterocyclic Chromophores. Indole and Purine Derivatives of Biological Significance

    Författare :Bo Albinsson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; linear dichroism LD ; fluorescence polarization anisotropy FPA ; electronic excitations; magnetic circular dichroism;

    Sammanfattning : Proteins and nucleic acids absorb light in the near-UV due to the presence of aromatic heterocyclic chromophores. In this work linear dichroism (LD) in combination with fluorescence polarization anisotropy (FPA) and magnetic circular dichroism (MCD) is used to investigate electronic excitations and excited states of indole derivatives, purine derivatives, the wye base, 2-phenylindole and 4',6-diamidino-2-phenylindole (DAPI). LÄS MER

  4. 3. Structural studies of bio-molecules and lipid assemblies using polarized light spectroscopy

    Författare :Maxim Kogan; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; linear dichroism; lipid membrane; spectroscopy; ruthenium complex; phospholipids; DNA breathing;

    Sammanfattning : The focus of the research presented in this thesis is structural studies of biological macromolecules using Linear Dichroism (LD) spectroscopy. The first part covers the flow alignment and structure of DMPC/DHPC phospholipid aggregates. LÄS MER

  5. 4. Polarized light spectroscopy studies of model membranes and DNA

    Författare :Maxim Kogan; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; emission; ruthenium dipyridophenazine complex; lipid bilayer; linear dichroism; membrane model; membrane orientation; bimolecular probe; DNA;

    Sammanfattning : Over the past decades Linear Dichroism (LD) spectroscopy has proven to be a powerful tool for studies of structure and functioning of different cell constituents, including DNA, cell membranes and proteins. Due to structural complexity of cell constituents, the LD studies of biomolecules depend on development of simplified model systems, which can mimic the original structure and functionality of the cell components. LÄS MER

  7. 5. DNA Interactions with Chiral Polyaza-aromatic Ruthenium(II) Complexes

    Författare :Per Lincoln; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; phenanthroline; binding geometry; binding isotherm; DNA; dppz; linear dichroism; intercalation; ruthenium; cooperative binding;

    Sammanfattning : The binding to DNA of a series of chiral ruthenium complexes is studied with optical spectroscopy techniques, including isotropic absorption, linear dichroism (LD) and emission anisotropy as well as steady-state and time-resolved luminescence. The thesis focuses on the ìlight-switchî complex [Ru(phen)2dppz]2+ which binds to DNA by intercalation and luminesces when bound to DNA but not in pure buffer solution (phen=1,10-phenanthroline; dppz=dipyrido[3,2-a:2',3'-c]phenazine). LÄS MER