Sökning: "limited proteolysis"

Visar resultat 1 - 5 av 21 avhandlingar innehållade orden limited proteolysis.

  1. 1. Methods for elucidating membrane protein structure and function

    Författare :Carolina Trkulja; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; patch clamp; antibodies; mass spectrometry; ion channels; proteomics; fluorescence; TRPV1; cholesterol.; limited proteolysis; Membrane proteins;

    Sammanfattning : Elucidation of the structure-function relationship of membrane proteins increases our understanding of many biological processes, both physiological and pathological. However, membrane proteins have been notoriously difficult to study, having part of their structure embedded into the lipid cell membrane. LÄS MER

  3. 2. On methods for probing structure and function of membrane proteins

    Författare :Erik Jansson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; proteomics; patch-clamp; mass spectrometry.; proteoliposomes; microfluidic superfusion; cholesterol; TRPV1; limited proteolysis; Membrane proteins; fluorescence;

    Sammanfattning : The determination of structure–function relationships in membrane proteins plays a key role for the understanding of molecular mechanisms in biological systems, and may provide information on how these systems can be modulated by pharmaceuticals. The aim of the work described herein was to develop methods for studying structure and function of membrane proteins, ideally in combination. LÄS MER

  4. 3. The cell biology and catalytic properties of the nutrient-induced signaling endoprotease Ssy5

    Författare :António Martins; Per O. Ljungdahl; Bruno André; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Nutrient sensing; signal transduction; regulated proteolysis; endoprotease; serine protease; substrate specificity; subcellular fractionation; Saccharomyces cerevisiae; Cell Biology; cellbiologi;

    Sammanfattning : Cells continuously sense and respond to changes in the presence, quality and quantity of external and internal nutrients. Specific signaling proteases have been identified based on their roles in processing or destruction of distinct sets of downstream effector proteins in response to environmental cues. LÄS MER

  5. 4. The insulin-like growth factor system : effects of circulating proteases

    Författare :Sara Gustafsson; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : The insulin-like growth factor-I and -II (IGF-I/-II) are peptide hormones important for growth and metabolism. They stimulate cell proliferation and differentiation and inhibit apoptosis. In addition, they have insulin-like effects on glucose and amino acid metabolism. LÄS MER

  7. 5. Production and engineering of a xyloglucan endo-transglycosylase from Populus tremula x tremuloides

    Författare :Maria Henriksson; Tuula Teeri; KTH; []
    Nyckelord :LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; xyloglucan endo-transglycosylase; retaining glycoside hydrolase; glycosynthase; Pichia pastoris; fed-batch fermentation; expanded-bed adsorption; Plant biotechnology; Växtbioteknik;

    Sammanfattning : The aim of this work was to develop a production process for the enzyme xyloglucan endo-transglycosylase from Populus tremula x tremuloides (PttXET16-34). The natural transglycosylating activity of this enzyme has previously been employed in a XET-Technology. LÄS MER