Sökning: "ligation level"

Visar resultat 1 - 5 av 37 avhandlingar innehållade orden ligation level.

  1. 1. Proximity Ligation and Barcoding Assays : Tools for analysis of proteins and protein complexes

    Författare :Di Wu; Masood Kamali-Moghaddam; Ulf Landegren; Ola Söderberg; Jörg Hoheisel; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proximity ligation assay; proximity barcoding assay; sensitive; in situ; multiplex; profiling; amyloid-beta; MARK; tau; CSF; biomarker; protein complexes; Alzheimer’s disease; amyotrophic lateral sclerosis; Molekylär medicin; Molecular Medicine;

    Sammanfattning : Proteins are fundamental structural, enzymatic and regulatory components of cells. Analysis of proteins, such as by measuring their concentrations, characterizing their modifications, and detecting their interactions, provides insights in how biological systems work physiologically or pathologically at the molecular level. LÄS MER

  3. 2. Development and Application of Triple Specific Proximity Ligation Assays (3PLA)

    Författare :Edith Schallmeiner; Ulf Landegren; Larry Gold; Uppsala universitet; []
    Nyckelord :Molecular medicine; proxmity ligation; method development; cell siganling; Molekylärmedicin;

    Sammanfattning : After the completion of the human genome project the human genome was annotated with the surprisingly small amount of 24 000 (www.ensemble.com) genes. LÄS MER

  4. 3. Surgery for rectal cancer : the impact of perioperative factors

    Författare :Daniel Kverneng Hultberg; Martin Rutegård; Jörgen Rutegård; Peter Matthiessen; Markku Haapamäki; Lydrup Marie-Louise; Tom Öresland; Umeå universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Rectal cancer; abdominal surgery; anastomotic leakage; anastomotic dehiscence; postoperative complications; vascular tie; ligation level; oncologic outcome; surgical oncology; survival; recurrence; functional outcome; urogenital; anorectal; incontinence; NSAID; COX; Surgery; kirurgi;

    Sammanfattning : Rectal cancer is one of the most common and deadly cancer forms worldwide. A large proportion of rectal cancer patients are surgically treated with curative intention, with anterior resection being the most frequently used method today. LÄS MER

  5. 4. High Content Analysis of Proteins and Protein Interactions by Proximity Ligation

    Författare :Karl-Johan Leuchowius; Ola Söderberg; Ulf Landegren; Paul Goldsmith; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; in situ; proximity ligation; flow cytometry; high content screening; rolling circle amplification; in situ PLA; single-cell; single-molecule; protein interactions; drug screening; post-translational modifications; Molecular medicine; Molekylär medicin; Molekylär medicin; Molecular Medicine;

    Sammanfattning : Fundamental to all biological processes is the interplay between biomolecules such as proteins and nucleic acids. Studies of interactions should therefore be more informative than mere detection of expressed proteins. Preferably, such studies should be performed in material that is as biologically and clinically relevant as possible, i.e. LÄS MER

  7. 5. Application of Proximity Ligation Assay for Multidirectional Studies on Transforming Growth Factor-β Pathway

    Författare :Agata Zieba; Ola Söderberg; Kathleen C. Flanders; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proximity ligation; TGF-β signaling; Smad; single cell analysis; cell cycle; context-dependent signaling; CRC; Molekylär cellbiologi; Molecular Cellbiology; Medicinal Chemistry; Läkemedelskemi; Molecular Biology; Molekylärbiologi;

    Sammanfattning : A comprehensive understanding of how the body and all its components function is essential when this knowledge is exploited for medical purposes. The achievements in biological and medical research during last decades has provided us with the complete human genome and identified signaling pathways that governs the cellular processes that facilitates the development and maintenance of higher order organisms. LÄS MER