Sökning: "library screening"

Visar resultat 1 - 5 av 125 avhandlingar innehållade orden library screening.

1. 1. CRISPR based technologies for high-throughput metabolic engineering of yeast

   Författare :Christos Skrekas; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; cloning; Saccharomyces cerevisiae; enrichment; metabolic engineering; selection; library; screening; CRISPR; directed evolution;

   Sammanfattning : The yeast Saccharomyces cerevisiae is a commonly used microorganism for metabolic engineering applications since it has a very well-studied metabolism and it can be easily genetically modified. This led to its use as a cell factory for the production of a wide variety of industrially relevant chemicals such as fuels, cosmetics and food additives. LÄS MER

3. 2. Combinatorial Methodology. Screening for affinity specific ligands to biological and artificial receptors

   Författare :Margareta Krook; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; peptide library; combinatorial; screening; phage display; affinity; DNA-interaction; chymotrypsin; peptide inhibitor; antibody; IgG; Fc; molecular imprinting; steroid; receptor; Biochemistry; Metabolism; Biokemi; Biotechnology; Bioteknik;

   Sammanfattning : Combinatorial screening methodology has been employed to select new affinity specific ligands to bilogical and artificial receptors. The screening methodology, in particular screening of combinatorial phage display peptide libraries against target molecules differing in function and shape, is focused upon. LÄS MER

4. 3. Molecular Screening for Target Discovery in Cancer

   Författare :Mårten Fryknäs; Anders Isaksson; Ulf Pettersson; Hugh Salter; Uppsala universitet; []
   Nyckelord :Genetics; Cancer; Drug resistance; Microarray; High-throughput screening; Genetik;

   Sammanfattning : Cancer is one of the major causes of death in the western world. Resistance to anti-cancer drugs and diagnostic difficulties are major obstacles to successful treatment. LÄS MER

5. 4. New Paradigms in GPCR Drug Discovery : Structure Prediction and Design of Ligands with Tailored Properties

   Författare :Mariama Jaiteh; Jens Carlsson; David E Gloriam; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; G Protein-Coupled Receptor; Molecular Docking; Virtual Screening; Homology Modeling; Molecular Dynamics Simulation; Chemical Library; Functionally Selective Ligand; Polypharmacology; Dimerization; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

   Sammanfattning : G protein-coupled receptors (GPCRs) constitute a large superfamily of membrane proteins with key roles in cellular signaling. Upon activation by a ligand, GPCRs transduce signals from the extracellular to the intracellular environment. LÄS MER

7. 5. Molecular Recognition in Antibody Engineering. Studies on recombinant and phage displayed antibodies

   Författare :Ann-Christin Malmborg Hager; Institutionen för immunteknologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; transplantation; affinity; antibody; kinetic; Immunologi; serologi; serology; Immunology; Bioteknik; Biotechnology; library; selection; surface display; phage display; association rate; dissociation rate;

   Sammanfattning : In vitro generation of antibody fragments of desired specificity and affinity plays an important role since it permits the generation of reagents which may be valuable for diagnostic and therapeutic applications. Furthermore, the kinetic parameters for an antibody-antigen interaction, rather than the affinity, has shown to correlate with biological functions like virus neutralization. LÄS MER