Sökning: "klenow"

Visar resultat 1 - 5 av 8 avhandlingar innehållade ordet klenow.

  1. 1. Protein based approaches for further development of the pyrosequencing technology platform

    Författare :Maria Ehn; KTH; []
    Nyckelord :pyrosenquencing; ssb; zbasic; klenow; apyrase; expression; purification;

    Sammanfattning : The innovation of DNA analysis techniques has enabled arevolution in the field of molecular biology. In the 70’s,first technologies for sequence determination of DNA wereinvented and these techniques enormously increased thepossibilities of genetic research. LÄS MER

  3. 2. Effects of fusion tags on protein partitioning In aqueous two-phase systems and use in primary protein recovery

    Författare :Cynthia Hassinen; KTH; []
    Nyckelord :E. coli; Aqueous two-phase systems; Fusion proteins; Hydrophobic interaction chromatography; Expanded bed adsorption; Beta-galactosidase; Klenow polymerase; Z-domain; Peptide tags;

    Sammanfattning : The two techniques aqueoustwo-phase partitioning and expanded bed adsorption that bothare suitable for primary protein recovery were studied. Most ofthe work was focused on partition in aqueous two-phase systemsand in particular on the possibility to effect the partitionbehaviour by fusion of short peptide tags or protein domains tothe target protein. LÄS MER

  4. 3. Advancements in Firefly Luciferase-Based Assays and Pyrosequencing Technology

    Författare :Jonas Eriksson; KTH; []
    Nyckelord :bioluminescence; osmolytes; glycine betaine; thermostability; firefly luciferase; inorganic pyrophosphatase; inorganic pyrophosphate; Pyrosequencing technology; secondary DNA-structures; Sequenase; Klenow-polymerase; reaction rates; temperature;

    Sammanfattning : Pyrosequencing is a new DNA sequencing method relying on thesequencing-by-synthesis principle and bioluminometric detectionof nucleotide incorporation events. The objective of thisthesis was improvement of the Pyrosequencing method byincreasing the thermal stability of firefly luciferase, and byintroducing an alternative DNA polymerase and a new nucleotideanalog. LÄS MER

  5. 4. Mathematical modelling and analysis of the pyrosequencing reaction system

    Författare :Anna Svantesson; Jeanette Hellgren Kotaleski; Anders Lansner; Pål Nyrén; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; apyrase; bioluminescence; DNA polymerase; DNA sequencing; enzyme kinetics; Klenow fragment; mathematical modelling; Michaelis-Menten; pyrosequencing; Computer science; Datalogi;

    Sammanfattning : .... LÄS MER

  7. 5. Protein Engineering by Directed Evolution and Rational Design

    Författare :Torbjörn Gräslund; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; ion-exchange chromatography; subtilisin; Klenow DNA polymerase; Taq DNA polymerase; directed evolution; rational design; charge engineering; phosphonylating inhibitor; phage display; circular dichroism; protein A; Bioengineering; Bioteknik;

    Sammanfattning : .... LÄS MER