Sökning: "kinetic proofreading"

Hittade 2 avhandlingar innehållade orden kinetic proofreading.

1. 1. Exploring and predicting DNA template dependent variation in transcription

   Författare :Harriet Mellenius; Måns Ehrenberg; Johan Elf; Dan Andersson; Otto Berg; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; transcription; accuracy; kinetic proofreading; selection; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

   Sammanfattning : Reliable transmission of information from DNA to proteins is a pre-requisite for all life, where substitution errors in the polypeptide chain may arise from transcription, aminoacylation of tRNAs or translation. The fidelity control mechanisms in transcription have nevertheless received little attention, based on the assumption that the transcriptional error is masked by the translational error. LÄS MER

3. 2. Accuracy of mRNA Translation in Bacterial Protein Synthesis

   Författare :Jingji Zhang; Måns Ehrenberg; Olke C. Uhlenbeck; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein synthesis; genetic code; misreading; error hot spots; kinetics; aminoglycoside; Biokemi; Biochemistry;

   Sammanfattning : Reading of messenger RNA (mRNA) by aminoacyl-tRNAs (aa-tRNAs) on the ribosomes in the bacterial cell occurs with high accuracy. It follows from the physical chemistry of enzymatic reactions that there must be a trade-off between rate and accuracy of initial tRNA selection in protein synthesis: when the current accuracy, the A-value, approaches its maximal possible value, the d-value, the kinetic efficiency of the reaction approaches zero. LÄS MER