Sökning: "isotope labeling"

Visar resultat 1 - 5 av 17 avhandlingar innehållade orden isotope labeling.

1. 1. Isotope labeling of proteins for spectroscopic studies

   Författare :Emil Gustavsson; Göteborgs universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Isotope labeling; Proteins; Cell-free protein expression; NMR; IR; Infrared;

   Sammanfattning : Protein structural dynamics have been closely tied to the molecular mechanisms controlling for example enzyme activities. In my work, I have mainly studied the bacteriophytochrome from Deinococcus radiodurans. LÄS MER

3. 2. Molecular dynamics studied by NMR relaxation experiments. Characterization of functional dynamics in the FK506 binding protein FKBP12

   Författare :Ulrika Brath; Biofysikalisk kemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; nuclear magnetic resonance; NMR; FK506; protein dynamics; chemical exchange; spin relaxation; FKBP12; FKBP; isotope labeling;

   Sammanfattning : The presented thesis work is concerned with the study of molecular dynamics using liquid nuclear magnetic resonance (NMR) spectroscopy. Spin relaxation dispersion experiments in the rotating frame (R1ρ) are employed to provide a detailed, atomic-resolution view of protein dynamics. LÄS MER

4. 3. Combined structural methods to characterize an oligomeric small heat shock protein Hsp21

   Författare :Gudrun Rutsdottir; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Small heat shock proteins; chaperones; crosslinking mass spectrometry; Stable isotope labeling; quantification; small angle X-ray scattering; cryo-electron microscopy; combined structural methods; plant stress;

   Sammanfattning : Small heat shock proteins (sHsps) are found in all organisms and evolved early in the history of life, prior to the divergence of the three domains of life (Archaea, Bacteria, and Eukarya). Their malfunction is connected to several different diseases in humans, such as Alzheimer’s and Parkinson’s disease. LÄS MER

5. 4. On the Ring-Opening Reaction of Thiophene-1,1-dioxides and the Intramolecular Diels-Alder Reaction of Azanona- and AzadecatrienesI

   Författare :Anders Sebastian Tsirk; Centrum för analys och syntes; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; transition states; 3-21G * ; intramolecular Diels-Alder reactions; asymmetric induction; multivariate optimization; kinetic isotope effects; rearrangements; secondary amines; 1-dioxides; Ring-opening reaction; thiophene-1; stereoselectivity.; Organic chemistry; Organisk kemi;

   Sammanfattning : Substituted thiophene-1,1-dioxides were synthesized and their ring-opening reactions with omega-unsaturated secondary amines were investigated; this led to a synthetic route to azatrienes and to the preparation of some polyhydroisoindoles and polyhydroisoquinolines via an intramolecular Diels-Alder reaction. By using 2-substituted pyrrolidines and piperidines various tetrahydrobenzo[f]indolizidines, benzo[b]quinolizidines, tetrahydrobenzo[a]indolizidines and tetrahydro[a]pyrrolizidines could be synthesized via the ring-opening of 3-bromo-2,5-dimmethylthiophene-1,1-dioxide and a subsequent intramolecular Diels-Alder reaction. LÄS MER

7. 5. Production and biomedical use of non-conventional positron emitters

   Författare :Vladimir Tolmachev; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Oncology; Onkologi; Oncology; Onkologi; Biomedical Radiation Science; biomedicinsk strålningsvetenskap;

   Sammanfattning : Positron Emission Tomography (PET) is a powerful diagnostic tool. So far, it has mainly been associated with the use of short-lived positron emitters with half-lives shorter than 2 h, like 11C and 18F. LÄS MER