Sökning: "intracellular sorting"

Visar resultat 6 - 10 av 30 avhandlingar innehållade orden intracellular sorting.

1. 6. Droplet microfluidics for screening and sorting of microbial cell factories

   Författare :Sara Björk; Helene Svahn Andersson; Håkan Jönsson; Christoph Merten; KTH; []
   Nyckelord :Droplet microfluidics; Cell factories; High-throughput screening; Cell culture; Bioteknologi; Biotechnology;

   Sammanfattning : Cell factories are cells that have been engineered to produce a compound of interest, ranging from biopharmaceuticals to biofuels. With advances in metabolic engineering, the number of cell factory variants to evaluate has increased dramatically, necessitating screening methods with increased throughput. LÄS MER

3. 7. Amyloid Beta – Biotherapy Target and Biotechnological Tool

   Författare :Sebastian W. Meister; Stefan Ståhl; John Löfblom; Maarten Merkx; KTH; []
   Nyckelord :Alzheimer’s disease; amyloid beta; blood-brain barrier; green-fluorescent protein; intracellular assays; proteases; protease substrate specificity; protein aggregation; protein engineering.; Bioteknologi; Biotechnology;

   Sammanfattning : Alzheimer’s disease is a progressive neurodegenerative disease characterized primarily by memory impairment and cognitive decline. As of 2020, an estimated 50 million people suffer from Alzheimer’s disease or related dementia and no disease-modifying treatment options are yet approved for clinical practice. LÄS MER

4. 8. Intracellular systems for characterization and engineering of proteases and their substrates

   Författare :George Kostallas; Stefan Ståhl; Patrik Samuelson; Matthew DeLisa; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; proteases; flow cytometry; GFP; CAT; protein engineering; ssrA; ClpXP; tobacco etch virus; TEV; site-specific; high-throughput; selection; proteolysis; libraries; Bioengineering; Bioteknik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Sammanfattning : Over the years, the view on proteases as relatively non-specific protein degradation enzymes, mainly involved in food digestion and intracellular protein turnover, has shifted and they are now recognized as key regulators of many biological processes that determine the fate of a cell. Besides their biological role, proteases have emerged as important tools in various biotechnical, industrial and medical applications. LÄS MER

5. 9. Biosynthesis, Processing, and Sorting of Human Neutrophil Granule Proteins. Focus on Cathepsin G, Proteinase 3, and Azurocidin

   Författare :Daniel Garwicz; Avdelningen för hematologi och transfusionsmedicin; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Hematologi; extracellular fluids; Haematology; differentiation; bone marrow progenitor cells; preproform; mutations; Cell lines; transfection; extracellulära vätskor;

   Sammanfattning : The neutrophil granulocyte contains a number of multifunctional proteins stored in different subsets of granules. The azurophil granules, synthesized early during differentiation, harbour leukocyte elastase, cathepsin G, proteinase 3, and azurocidin; the members of the serprocidin family. LÄS MER

7. 10. Targeting of viral proteins to axons and dendrites

   Författare :Katarzyna Weclewicz; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : In the thesis distribution of different proteins of enveloped and non-enveloped viruses was studied in neurons, which are polarized cells with an axonal and a somatodendritic domain. Such studies may give better insight not only into virus spread within neuronal populations and selective changes in infected neurons as a consequence of the spread, but they may also contribute to an understanding of the basic sorting mechanisms, which are responsible for asymmetric targeting of macromolecules to the pre- and postsynaptic regions in a nerve cell. LÄS MER