Sökning: "integrase"

Visar resultat 1 - 5 av 21 avhandlingar innehållade ordet integrase.

  1. 1. Structure-Function Studies of Bacteriophage P2 Integrase and Cox protein

    Författare :Jesper Eriksson; Elisabeth Haggård; Karin Hammer; Stockholms universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Bacteriophage P2; site-specific recombination; integrase; transcriptional switch; Genetics; Genetik;

    Sammanfattning : Probably no group of organisms has been as important as bacteriophages when it comes to the understanding of fundamental biological processes like transcriptional control, DNA replication, site-specific recombination, e.t.c.The work presented in this thesis is a contribution towards the complete understanding of these organisms. LÄS MER

  2. 2. Site-specific recombination of P2-like phages; possible tools for safe gene therapy : A focus on phage ΦD145

    Författare :Sridhar Mandali; Elisabeth Haggård-Ljungquist; Richard Calendar; Stockholms universitet; []
    Nyckelord :bacteriophage; integrase; site-specific recombination; NATURAL SCIENCES; NATURVETENSKAP; molekylärgenetik; Molecular Genetics;

    Sammanfattning : P2-like bacteriophages integrate their genome into the E. coli host cell by a site-specific recombination event upon lysogenization. The integrative recombination occurs between a specific sequence in the phage genome, attP, and a specific sequence in the host genome, attB, generating the host-phage junctions attL and attR. LÄS MER

  3. 3. Computational Modelling of Ligand Complexes with G-Protein Coupled Receptors, Ion Channels and Enzymes

    Författare :Lars Boukharta; Johan Åqvist; Christopher A. Reynolds; Uppsala universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; NATURAL SCIENCES; computer simulations; molecular dynamics; ligand binding; free energy perturbation; linear interaction energy; binding free energy; homology modelling; structure prediction; alanine scanning; site-directed mutagenesis; hERG; GPCR; neuropeptide Y; HIV-1 reverse transcriptase; integron integrase; Molecular Biotechnology; Molekylär bioteknik;

    Sammanfattning : Accurate predictions of binding free energies from computer simulations are an invaluable resource for understanding biochemical processes and drug action. The primary aim of the work described in the thesis was to predict and understand ligand binding to several proteins of major pharmaceutical importance using computational methods. LÄS MER

  4. 4. Functional characterization of a phage integrase and its possible use in gene therapy

    Författare :Clara Frumerie; Elisabeth Haggård-Ljungquist; Lars Sundström; Stockholms universitet; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Clinical genetics; Klinisk genetik;

    Sammanfattning : Bacteriophage P2 infecting Escherichia coli can integrate into the bacterial chromosome by site-specific recombination, which is catalyzed by the P2 Int recombinase. The recombination event takes place between the phage attachment site, attP, and the bacterial attachment site, attB. LÄS MER

  5. 5. Palladium-Catalysed Couplings in Organic Synthesis : Exploring Catalyst-Presenting Strategies and Medicinal Chemistry Applications

    Författare :Alejandro Trejos; Mats Larhed; Jianliang Xiao; Uppsala universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Mizoroki-Heck arylation; Suzuki coupling; domino reaction; stereoselective; palladium; chelation control; vinyl ether; HIV-protease inhibitors; HIV-integrase inhibitors; Kemi med inriktning mot organisk kemi; Chemistry with specialization in Organic Chemistry;

    Sammanfattning : Palladium-catalysed coupling reactions have been embraced by synthetic chemists as one of the preferred means for smooth formation of new carbon-carbon bonds: a truly ubiquitous methodology of synthesizing complex molecules.This thesis describes the study of a series of palladium(0)-catalysed C2-arylations of a 1-cyclopentenyl ether, equipped with a chiral (S)-N-methyl-pyrrolidine auxiliary. LÄS MER