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Visar resultat 1 - 5 av 9 avhandlingar som matchar ovanstående sökkriterier.

  1. 1. Diffusive interactions play an important role in protein stability and mobility : Investigations of the intracellular milieu using in-cell NMR

    Författare :Therese Sörensen; Mikael Oliveberg; Per Jemth; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; in-cell NMR; protein-protein interactions; mobility; stability; thermodynamics; Nuclear Magnetic Resonance; stopped-flow spectroscopy; proteins; SOD1; HAH1; TTHA1718; cells; cytoplasm; humans; bacteria; ions; polyions; electrostatics; biokemi; Biochemistry;

    Sammanfattning : Proteins are crucial for all cellular life. Every signal received by a cell, and every response to it, is mediated by proteins. Inside cells, these proteins diffusively sample each other’s surfaces, as they travel through the cytoplasm in search of their specific interaction partners. LÄS MER

  3. 2. Biophysical chemistry of the ALS-associated protein SOD1 : Implications for folding, aggregation and in-cell behaviour

    Författare :Fan Yang; Mikael Oliveberg; Per Jemth; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; SOD1 ALS folding in-cell NMR aggregation; Biochemistry; biokemi;

    Sammanfattning : Biophysical chemistry deals with the structural behavior, properties and molecular function of biological macromolecules. A long-standing challenge is here to establish how these macromolecular features change upon transfer from simplified conditions in vitro to the crowded and molecularly complex environment of live cells. LÄS MER

  4. 3. How transient interactions in the crowded cytosol affect protein mobility and stability

    Författare :Sarah Leeb; Mikael Oliveberg; Magnus Wolf-Watz; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; diffusive transient interactions; macromolecular crowding; protein stability; in-cell NMR; protein evolution; Biophysics; biofysik;

    Sammanfattning : Most biochemical reactions have evolved in crowded intracellular environments. However, the complexity of intracellular environments is often neglected in structural or functional studies of proteins. LÄS MER

  5. 4. Protein stability and mobility in live cells : Revelation of the intracellular diffusive interaction organization mechanisms

    Författare :Xin Mu; Mikael Oliveberg; Johan Elf; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; diffusive interactions; macromolecular crowding; protein thermodynamic stability; protein mobility; in-cell NMR; Hofmeister series; Biochemistry; biokemi;

    Sammanfattning : Biochemical processes inside living cells take place in a confined and highly crowded environment. As such, macromolecular crowding, one of the most important physicochemical properties of cytoplasm, is an essential element of cell physiology. LÄS MER

  7. 5. Effect of Ischaemia-Reperfusion on Rabbit Kidney and Human Brain

    Författare :Jagdish Shivlal Gondalia; Göteborgs universitet; []
    Nyckelord :Free radicals; ischaemia-reperfusion; cell bioenergetics; NMR; ESR; carotid artery stenosis; endarterectomy; allopurinol; acetylcysteine; MMP-1; ICAM-1; MCP-9; oxLDL;

    Sammanfattning : Free radicals are produced in various organs at ischaemia-reperfusion. The final stage in radical damage is lipid peroxidation. We have demonstrated previously that a lipid-soluble antioxidant improves restoration of bioenergetics in rabbit kidneys after ischaemia, as reflected in 31P spectrometry. LÄS MER