Sökning: "in vivo transfection"

Visar resultat 1 - 5 av 62 avhandlingar innehållade orden in vivo transfection.

  1. 1. Characterization of nucleic acid delivery with fatty acid modified cell-penetrating peptide nanoparticle formulations

    Författare :Tõnis Lehto; Ülo Langel; Patrick Midoux; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Transfection; gene delivery; oligonucleotide; complexes; non-viral; in vivo; neurokemi med molekylär neurobiologi; Neurochemistry with Molecular Neurobiology;

    Sammanfattning : Recent advances with techniques used for manipulating gene expression have brought us to an era where various gene therapeutic approaches are becoming common therapeutic tools for many previously incurable diseases. The main factor impeding the wider translation of gene therapy is that the active pharmaceutical ingredients used for interfering with gene expression are based on nucleic acids and synthetic oligonucleotides and such molecules do not readily reach their intracellular targets due to their physicochemical properties and therefore they require delivery vectors to cross the cell membrane. LÄS MER

  3. 2. Phospholipase C β3, and its role in endocrine tumorigenesis

    Författare :Peter Stålberg; Uppsala universitet; []
    Nyckelord :Medical sciences; Phospholipase C beta 3; endocrine tumors; in situ hybridization; transfection; tumorsuppressor; differential expression; gene targeting; MEDICIN OCH VÅRD; MEDICINE; MEDICIN; Medicine; medicin;

    Sammanfattning : The Phospholipase C β3 (PLCB3) gene is located on chromosome 11q13, in the vicinity of theMultiple Endocrine Neoplasia type 1 (MEN1) suppressor gene. PLCB3 has been implicated inendocrine tumorigenesis based on findings of low expression in endocrine tumors, and the probableexistence of other suppressor genes located at 11q13. LÄS MER

  4. 3. RNA systems for NMR studies in vitro and in vivo

    Författare :Hannes Feyrer; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : NMR spectroscopy is an excellent tool to study the structure-function relationship of RNA. Such measurements are usually performed in vitro, which requires large amounts of isotope- labeled sample in high purity and can give access to individual atoms, structure of the molecule and conformational dynamics. LÄS MER

  5. 4. NEURAL STEM/PROGENITOR CELLS IN THE POST-ISCHEMIC ENVIRONMENT: Proliferation, Differentiation and Neuroprotection

    Författare :Jonas Faijerson; Göteborgs universitet; []
    Nyckelord :neural stem cells; neural progenitor cells; stroke; ischemia; reactive astrocytes; astrogliosis; proliferation; differentiation; neurogenesis; excitotoxicity; transfection; neuroprotection;

    Sammanfattning : Stroke is one of the leading causes of chronic disability and death in the Western world. Today, no treatment can repair the cellular loss associated with an ischemic lesion. LÄS MER

  7. 5. Profiling and exploiting lipid-based nanoparticles in vitro and in vivo

    Författare :Helena Sork; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : One of the major hurdles for therapeutic applications is the efficient delivery of bioactive molecules to the site of action. The high flexibility and biosafety of lipid-based nanoparticles has greatly enhanced their employment as delivery systems not only for synthetic but also for natural molecules such as proteins and nucleic acids. LÄS MER