Sökning: "in vivo probing"

Visar resultat 1 - 5 av 9 avhandlingar innehållade orden in vivo probing.

  1. 1. Lead(II) as a Tool for Probing RNA Structure in vivo

    Författare :Magnus Lindell; Gerhart Wagner; Roland Hartmann; Uppsala universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Molecular biology; lead II cleavage; RNA structure; in vivo probing; Molekylärbiologi; Molecular biology; Molekylärbiologi;

    Sammanfattning : Chemical modification and limited enzymatic hydrolysis are powerful methods to obtain detailed information on the structure and dynamics of RNAs in solution. In the work presented here I have taken advantage of the properties of the divalent metal ion lead(II) to establish it as a new probe for investigating the structure of RNA in vivo. LÄS MER

  2. 2. Molecular probes as protein analysis tools in biotechnology

    Författare :Anngelica Jarl; Linköpings universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP;

    Sammanfattning : Protein-labeling today is a work of art, in vivo studies of proteins or other molecules can easily be performed, and the movement of the labeled molecule can be followed in real time. Labeling in vitro gives enormous amount of data in labs all over the world on a daily basis, where protein-protein, protein-DNA or other interactions are studied. LÄS MER

  3. 3. PROBING NAD(P)H AVAILABILITY IN LIVING CELLS USING MINIATURIZED ELECTROCHEMICAL SYSTEMS

    Författare :Natalie Kostesha; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; redox; NAD P H; living cells; S. cerevisiae; mediated amperometry; microelectrode; electrochemistry;

    Sammanfattning : The work presented in this thesis demonstrates the application of electrochemical methods for probing the dynamics of cellular metabolism in prokaryotic and eukaryotic cells. The main part of the work has been done using genetically modified strains of yeast. Employing baker’s yeast, S. LÄS MER

  4. 4. In Vitro Kinetics of Ribosomal Incorporation of Unnatural Amino Acids

    Författare :Jinfan Wang; Anthony Forster; Jason Chin; Uppsala universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; ribosome; protein synthesis; unnatural amino acids; kinetics; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi;

    Sammanfattning : Ribosomal incorporation of unnatural amino acids (AAs) into peptides or proteins has found broad applications in studying translation mechanism, discovering potential therapeutics, and probing protein structure and function. However, such applications are generally limited by the low incorporation efficiencies of the unnatural AAs. LÄS MER

  5. 5. Deciphering the role of 27-hydroxycholesterol in neurodegeneration

    Författare :Muhammad Al Mustafa Ismail; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP;

    Sammanfattning : Cholesterol is constantly attributed to neurodegenerative diseases including Alzheimer’s disease (AD). This is due to the association of high blood cholesterol and the consequences of cholesterol metabolism in these conditions. LÄS MER