Sökning: "in vitro motility assay"
Visar resultat 1 - 5 av 12 avhandlingar innehållade orden in vitro motility assay.
1. Actomyosin in biocomputation
Sammanfattning : There exist complex mathematical problems that are important in real world applications such as weather prediction, molecular modelling, network route optimization and more. In general, such problems are solved using supercomputers with higher computing efficiency but this also consumes high energy along with high production and maintenance cost. LÄS MER
2. Characterization and optimization of the in vitro motility assay for fundamental studies of myosin II
Sammanfattning : Myosin II is the molecular motor responsible for muscle contraction. It transforms the chemical energy in ATP into mechanical work while interacting with actin filaments in so called cross-bridge cycles. Myosin II or its proteolytic fragments e.g. LÄS MER
3. Biophysical studies of the actin-myosin motor system and applications in nanoscience
Sammanfattning : The actin-myosin motor system plays important roles in cellular processes. In addition, actin and myosin have been used for developments towards nanotechnological applications in recent years. LÄS MER
4. Celluar and Molecular Mechanisms Underlying Regulation of Skeletal Muscle Contraction in Health and Disease
Sammanfattning : Morphological changes, genetic modifications, and cell functional alterations are not always parallel. Therefore, assessment of skeletal muscle function is an integral part of the etiological approach. LÄS MER
5. A novel single skeletal muscle cell in vitro motility assay : effects of aging and non-enzymatic glycosylation on myosin function
Sammanfattning : The aims of this study were to develop a single skeletal muscle fiber in vitro motility assay, and to employ the assay in order to study the effects of aging and non-enzymatic glycosylation on the mechanical properties of the motor protein myosin in an attempt to improve our understanding of the molecular mechanisms underlying the aging-related impairment in regulation of muscle function. Myosin was extracted from a millimeter short single muscle fiber segment directly onto a nitrocellulosecoated coverslip for subsequent analyses of the functional properties of the motor protein. LÄS MER