Sökning: "in situ sequencing"
Visar resultat 1 - 5 av 85 avhandlingar innehållade orden in situ sequencing.
1. In situ Sequencing : Methods for spatially-resolved transcriptome analysis
Sammanfattning : It is well known that cells in tissues display a large heterogeneity in gene expression due to differences in cell lineage origin and variation in the local environment at different sites in the tissue, a heterogeneity that is difficult to study by analyzing bulk RNA extracts from tissue. Recently, genome-wide transcriptome analysis technologies have enabled the analysis of this variation with single-cell resolution. LÄS MER
2. Towards comprehensive cellular atlases : High-throughput cell mapping by in situ sequencing
Sammanfattning : With recent technological advancements in single-cell biology, many aspects of individual cells are characterized with unprecedented resolution and details. Cell types in human and model organisms are redefined, and multiple organ-wide atlases are proposed to integrate different types of data to provide a comprehensive view of biological systems at cellular resolution. LÄS MER
3. Detection and Sequencing of Amplified Single Molecules
Sammanfattning : Improved analytical methods provide new opportunities for both biological research and medical applications. This thesis describes several novel molecular techniques for nucleic acid and protein analysis based on detection or sequencing of amplified single molecules (ASMs). LÄS MER
4. Genotyping and Mutation Detection In Situ : Development and application of single-molecule techniques
Sammanfattning : The human body is composed of trillions of cells closely working together to maintain a functional organism. Every cell is unique in molecular composition and can acquire genetic variations that might cause it to turn pathological. LÄS MER
5. Development of Spatial Sequencing Methods and Applications for Brain Circuit Repair in Parkinson’s Disease
Sammanfattning : .... LÄS MER