Sökning: "in situ hybridization histochemistry"

Visar resultat 1 - 5 av 15 avhandlingar innehållade orden in situ hybridization histochemistry.

1. 1. Monoamine transporters in female human reproduction

   Författare :Barbara Bottalico; Lund Obstetrik och gynekologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; PMAT; VMAT2; OCT; NET; in-situ hybridization histochemistry; decidualization; human endometrium; placenta; Medicine human and vertebrates ; Monoamine transporters; preeclampsia; histamine; Medicin människa och djur ;

   Sammanfattning : The present study explored the gene and protein expression of the monoamine transporters in human endometrium throughout the menstrual cycle, in early decidua and in placentas from normal as well as preeclamptic pregnancies using in-situ hybridization, real time-PCR, immunohistochemistry and primary tissue cultures. Four distinguishable patterns were observed in the endometrium over the menstrual cycle: (1) epithelial expression of norepinephrine transporter (NET) mRNA, (2) Stromal co- expression of vesicular monoamine transporter 2 (VMAT2) and plasma membrane monoamine transporter (PMAT) mRNAs with maximal intensity in the proliferative phase; (3) increasing epithelial expression of VMAT2 mRNA with a maximum in the late secretory phase; (4) stromal expression of extra-neuronal monoamine transporter (EMT) mRNA with a peak in the early secretory phase. LÄS MER

3. 2. Distribution of serotonin receptors and transporters in the human brain : implications for psychosis

   Författare :Katarina Varnäs; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :5-HT receptors; autoradiography; in situ hybridization histochemistry; psychotic disorders;

   Sammanfattning : The serotonin (5-HT) system is thought to be involved in different psychiatric disorders, including depression and anxiety disorders, and is a major target for the pharmacological treatment of these conditions. The involvement of the 5-HT system in psychosis has been suggested, as 5-HT receptor agonism is a common mechanism of different classes of hallucinogenic drugs and several antipsychotics are 5-HT receptor antagonists. LÄS MER

4. 3. PLASMA PROTEINS IN NORMAL AND PREECLAMPTIC PREGNANCY

   Författare :Karl Kristensen; Lund Obstetrik och gynekologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; gynaecology; andrology; reproduction; sexuality; Obstetrik; Obstetrics; pregnancy; renal function; serum amyloid A protein; Clinical chemistry; Klinisk kemi; preeclampsia; beta-2 microglobulin; C-reactive protein; cystatin C; inflammation; placenta; beta trace protein; gynekologi; andrologi; reproduktion; sexualitet;

   Sammanfattning : The aim of the research presented in this thesis was to provide extended background knowledge concerning several biochemical markers, used for the medical evaluation of pregnant women with suspected or confirmed preeclampsia, in order to improve the reliability of monitoring. Plasma levels of beta-2 microglobulin, cystatin C and beta trace protein are known to reflect renal filtration in non-pregnant settings, and the plasma proteins, C-reactive protein and serum amyloid A protein, are known to be sensitive markers of inflammation. LÄS MER

5. 4. Defence capabilities of human intestinal epithelial cells

   Författare :Anna Fahlgren; Sten Hammarström; Marie-Louise Hammarström; Gudmundur Gudmundsson; Umeå universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Immunology; intestine; mucosa; epithelium; antimicrobial; defensins; glycocalyx; ulcerative colitis; Crohn´s disease; celiac disease; Immunologi; Immunology; Immunologi; immunologi; Immunology;

   Sammanfattning : The epithelial cells lining the intestinal mucosa separate the underlying tissue from components of the intestinal lumen. Innate immunity mediated by intestinal epithelial cells (IECs) provides rapid protective functions against microorganisms. Innate immunity also participates in orchestrating adaptive immunity. LÄS MER

7. 5. Chemokine receptor expression and function in experimental autoimmune neuroinflammation

   Författare :Sana Eltayeb; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Neuroinflammatory lesions in the central nervous system (CNS) are characterized by the presence of leukocytes, mainly monocytes/macrophages, derived from the systemic compartment. We believe CC inflammatory chemokine receptors (CkRs), CCR1, CCR2 and CCR5 are prerequisite for controlling the migration of monocytes/macrophages into inflammatory foci. LÄS MER