Sökning: "homogeneous hybridization assays"

Hittade 1 avhandling innehållade orden homogeneous hybridization assays.

  1. 1. The Light-up Probe

    Författare :Nicke Svanvik; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; asymmetric cyanine dyes; light-up probes; PNA; real-time PCR; LightUp Probe; fluorescent hybridization probes; homogeneous hybridization assays;

    Sammanfattning : The work presented in this thesis concerns the development of light-up probes and their potential applications in PCR based molecular diagnostics. The light-up probe is a nucleic acid analog (here PNA) oligomer linked to an asymmetric cyanine dye (here thiazole orange) and it combines the excellent hybridization properties of PNA with the large fluorescence enhancement of the dye upon binding to DNA. LÄS MER