Sökning: "high-throughput DNA analysis"

Visar resultat 1 - 5 av 80 avhandlingar innehållade orden high-throughput DNA analysis.

1. 1. Droplet microfluidics for high throughput biological analysis

   Författare :Håkan Jönsson; Helene Andersson-Svahn; Andrew Griffiths; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Microfluidics; Droplet microfluidics; High throughput biology; Single cell analysis; Hydrodynamic separation; Enzyme amplification; Fluorescence polarization; Microfabrication; Bioengineering; Bioteknik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Sammanfattning : Many areas of biological research increasingly perform large-scale analyses.  In genomics the entire gene repertoire of an organism is analyzed.  Proteomics attempts to understand the function and expression patterns of all proteins in a cell or organism. LÄS MER

3. 2. Improved Techniques for High-Throughput Molecular Diagnostics

   Författare :Mario Curcio; KTH; []
   Nyckelord :DNA; Molecular Diagnostics;

   Sammanfattning : The amount of information derived from sequencing the humangenome is leading to an exponential increase in the rate atwhich genes and genetic disorders are mapped and characterized.As a consequence, the demand for genetic testing is alsodramatically increasing. LÄS MER

4. 3. Improved statistical methodology for high-throughput omics data analysis

   Författare :Wenjiang Deng; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Over the last two decades, the advent of high-throughput omics technology has substantially revolutionized biological and biomedical research. A large volume of omics data has been produced with the rapid development of sequencing techniques. LÄS MER

5. 4. Exploring genetic heterogeneity in cancer using high-throughput DNA and RNA sequencing

   Författare :Erik Fasterius; Cristina Al-Khalili Szigyarto; Mathias Uhlén; Peter-Bram ’t Hoen; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; Biotechnology; bioinformatics; RNA-seq; WGS; WES; systems biology; variant analysis; single nucleotide variant; gene expression; machine learning; clustering; open source; R; bioconductor; Python; Bioteknologi; Biotechnology;

   Sammanfattning : High-throughput sequencing (HTS) technology has revolutionised the biomedical sciences, where it is used to analyse the genetic makeup and gene expression patterns of both primary patient tissue samples and models cultivated in vitro. This makes it especially useful for research on cancer, a disease that is characterised by its deadliness and genetic heterogeneity. LÄS MER

7. 5. Droplet microfluidics for single cell and nucleic acid analysis

   Författare :Prem Kumar Periyannan Rajeswari; Helene Andersson Svahn; Håkan Jönsson; Martin Dufva; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Acoustophoresis; Biomarker detection; Cell behavior analysis; Cell factories; Droplet microfluidics; Droplet PCR; High throughput biology; Label-free enrichment; Single cell analysis; Bioteknologi; Biotechnology;

   Sammanfattning : Droplet microfluidics is an emerging technology for analysis of single cells and biomolecules at high throughput. The controlled encapsulation of particles along with the surrounding microenvironment in discrete droplets, which acts as miniaturized reaction vessels, allows millions of particles to be screened in parallel. LÄS MER