Sökning: "high throughput screening model"

Visar resultat 6 - 10 av 55 avhandlingar innehållade orden high throughput screening model.

1. 6. microRNAs: from biogenesis to organismal tracing

   Författare :Wenjing Kang; Marc Friedländer; Jens Lagergren; Ulf Ørom; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; microRNA; microRNA biogenesis; taxonomical identification; high throughput sequencing; xenomiRs; RNA structure; high throughput screening; molekylär biovetenskap; Molecular Bioscience;

   Sammanfattning : MicroRNAs (miRNAs) are short noncoding RNAs of around 22 nucleotides in length, which help to shape the expression of most mRNAs. Perturbation of miRNA expression has revealed a variety of defects in development, cell specification, physiology and behavior. LÄS MER

3. 7. Methods for 2D and 3D Quantitative Microscopy of Biological Samples

   Författare :Amin Allalou; Carolina Wählby; Ida-Maria Sintorn; Ewert Bengtsson; Jens Rittscher; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Image analysis; cytmetry; model organism; zebrafish; screening; Computerized Image Processing; Datoriserad bildbehandling;

   Sammanfattning : New microscopy techniques are continuously developed, resulting in more rapid acquisition of large amounts of data. Manual analysis of such data is extremely time-consuming and many features are difficult to quantify without the aid of a computer. LÄS MER

4. 8. Sensitivity, Noise and Detection of Enzyme Inhibition in Progress Curves

   Författare :Omar Gutierrez Arenas; Helena Danielson; Athel Cornish-Bowden; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Progress curves; limit of detection; Z -factor; primary screening; enzyme inhibition; error structure; sensitivity analysis; High-throughput screning; Biokemi;

   Sammanfattning : Starting with the development of an enzymatic assay, where an enzyme in solution hydrolysed a solid-phase bound peptide, a model for the kinetics of enzyme action was introduced. This model allowed the estimation of kinetic parameters and enzyme activity for a system that has the peculiarity of not being saturable with the substrate, but with the enzyme. LÄS MER

5. 9. Strength in Numbers – Droplet Microfluidics for Multicellular Ensemble Applications

   Författare :Martin Trossbach; Aman Russom; Håkan Jönsson; Nicole Pamme; KTH; []
   Nyckelord :Droplet Microfluidics; High-Throughput Screening; Microtissues; Microreactors; Cell Factories; Cell Culture; Biomaterials; Spheroids; Biotechnology; Bioteknologi;

   Sammanfattning : The work presented in this doctoral thesis explores multicellular, biological, and biotechnological applications for microfluidic droplets, making use of a number of unique features of these miniaturized, highly scalable reaction vessels.Droplet microfluidics specializes in pico- to nanoliter sized aqueous droplets in an immiscible oil phase, borrowing techniques from the field of microfluidics, namely fluid actuation, detection systems and electronic peripherals. LÄS MER

7. 10. Neuron-to-neuron propagation of neurodegenerative proteins; relation to degradative systems

   Författare :Jakob Domert; Martin Hallbeck; Jan Marcusson; Lars Tjernberg; Linköpings universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cell-to-cell transfer; beta-amyloid; Alzheimer s disease; degradation; proteasome; alpha-synuclein; Parkinson s disease; high throughput screening model;

   Sammanfattning : Alzheimer’s disease (AD) and Parkinson’s disease (PD) are defined by neurodegeneration and accumulations of misfolded proteins that spread through the brain in a well characterized manner. In AD these accumulations consist mainly of β-amyloid (Aβ) and tau, while in PD, α-synuclein (α-syn) make up the characteristic lewy pathology. LÄS MER