Sökning: "heterologous"

Visar resultat 1 - 5 av 159 avhandlingar innehållade ordet heterologous.

  1. 1. Heterologous expression and characterization of xyloglucan endotransglycosylases from Populus tremula x tremuloides

    Författare :Åsa Kallas; KTH; []
    Nyckelord :xyloglucan endotransglycosylase; transglycosylation; hybrid aspen; cell expansion; heterologous expression; Pichia pastoris; N-glycosylation; X-ray crystallography;

    Sammanfattning : Xyloglucan endotransglycosylases (XETs) are enzymes involvedin the reorganization of the primary cell wall. This thesisdescribes several aspects of XET16A from hybrid aspen (Populustremula x tremuloides). The gene was isolated from the hybridaspen cDNA library and the protein was expressed heterologouslyin the methylotrophic yeast Pichia pastoris. LÄS MER

  3. 2. Laccases and Oxalate-Degrading Enzymes Heterologous Expression and Novel Applications in Lignocellulose Processing

    Författare :Pierre Cassland; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; virology; mycology; Mikrobiologi; virologi; mykologi; bacteriology; Microbiology; Lignocellulose Processing; Heterologous expression; Laccases; Oxalate-Degrading Enzymes; bakteriologi;

    Sammanfattning : Lignocellulose constitutes a vast renewable resource for the production of, for example, paper and bioethanol. The potential of using laccase and oxalate-degrading enzymes in novel applications for the processing of lignocellulose was investigated in this work. LÄS MER

  4. 3. Bench-Scale Production of Heterologous Proteins from Extremophiles- Escherichia coli and Pichia pastoris based expression systems

    Författare :Santosh Ramchuran; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Glycoside hydrolase; Heterologous protein; High cell density cultivation Expression systems; Bioteknik; Biotechnology; Fed-batch;

    Sammanfattning : Over the past few years considerable research attention has been assigned to extremophiles as sources of extremozymes due to their applicability in industrial processes, and the development of eco-friendly technologies. The establishment of efficient production strategies for heterologous proteins is an empirical process requiring broad background knowledge on available expression systems together with their major advantages and shortcomings. LÄS MER

  5. 4. Extracellular serine proteases as virulence factors in nematophagous fungi: Molecular characterization and functional analysis of the PII protease in Arthrobotrys oligospora

    Författare :Johan Åhman; Biologiska institutionen; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biology; Aspergillus niger.; Heterologous expression; Over-expression; Gene-disruption; Transformation system; Serine protease; Biological control; Arthrobotrys oligospora; Fungi; Nematophagous; Biologi;

    Sammanfattning : Nematophagous fungi are parasites of nematodes, thus they have been considered and tested for biological control of parasitic nematodes, but so far with limited success. Due to the fact that the nematode cuticle is composed of proteins it is likely that fungal extracellular proteases are involved in the infection of nematodes. LÄS MER

  7. 5. Evolution of mate signalling in moths: Biosynthetic gene families and diversification of female sex pheromones

    Författare :Marjorie Liénard; Biologiska institutionen; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; duplication; transcriptome; fatty-acyl-CoA reductase; fatty-acyl-CoA desaturase; fatty-acid biosynthesis; odour; insect; Lepidoptera; phylogeny; qPCR; heterologous expression; mutagenesis;

    Sammanfattning : Pheromones are intraspecific chemical signals serving as a ubiquitous form of communication, particularly among insects. Female sex-pheromone signals act as the core constituent in the specialized odour-mediated moth mate-recognition system. Female moths typically release multicomponent mixtures of alcohols, aldehydes or acetates. LÄS MER