Sökning: "heterologous expression"

Visar resultat 21 - 25 av 99 avhandlingar innehållade orden heterologous expression.

  1. 21. Expression and function of TRP channels in peripheral sensory neurons

    Författare :Susanne Staaf; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Transient receptor potential (TRP) channels comprise a family of multifunctional proteins which has since its discovery been of particular interest in sensory biology even though they are also frequently expressed in non-neuronal cell types. It is evident that TRP channels play an important role in the function of sensory neurons, not at least by being sensors of external stimuli. LÄS MER

  3. 22. Plant Aquaporins: A study of expression, localization, specificity, and regulation

    Författare :Sara Sjövall Larsen; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Växtbiokemi; Plant biochemistry; phosphorylation; protein kinase; overexpression; NIP; MIP; plant; aquaporin;

    Sammanfattning : Aquaporins, or Major Intrinsic Proteins (MIPs), are integral proteins that facilitate transport of water and other small neutral solutes across biological membranes. They belong to a well conserved and ancient family of proteins, present in all organisms ranging from bacteria to plants and humans. LÄS MER

  4. 23. Rational and combinatorial genetic engineering approaches for improved recombinant protein production and purification

    Författare :Nina Bandmann; Per-Åke Nygren; Helena Berglund; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Aqueous two-phase system; combinatorial library; expression vector; flow cytometry; fusion tag; partitioning; peptide library; phage display; recombinant proteins; ribosomal binding site; translation initiation; Bioengineering; Bioteknik;

    Sammanfattning : The bacterium Escherichia coli (E. coli) is in many situations an ideal host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve sufficiently high product quantities. LÄS MER

  5. 24. Functional genomics at the interface of protein expression and biophysical analysis

    Författare :Xiaoqiu Wu; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Protein characterization is central to any functional genomics effort, hence protein expression and purification play fundamental roles in these initiatives. In this study strategies for protein expression in E. coli were analysed, and several parameters were investigated respect to high-level expression of functional proteins. LÄS MER

  7. 25. In vitro and in vivo oxidation of diastereomers of lignin models of arylgylcerol b-aryl ether type and heterologous expression of laccase

    Författare :Christina Bohlin; Leif Jönsson; Ming Tien; Karlstads universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi; Kemi; Chemistry;

    Sammanfattning : Enzymic and non-enzymic oxidation of lignin models of the arylglycerol β-aryl ether type was investigated in experiments aimed at providing a better understanding of the initial phase of biological lignin degradation. Product profiles and diasteromer selectivity were determined to gain knowledge of the properties of the oxidants and pave the way for evaluation of the presence of the oxidants in ligninolytic fungal cultures. LÄS MER