Sökning: "green fluorescent protein."

Visar resultat 21 - 25 av 92 avhandlingar innehållade orden green fluorescent protein..

  1. 21. RNA-protein complexes as autoantigens : cell and molecular biology of Ro/SSA and disease associations of anti-Ro/SSA antibodies

    Författare :Nader Pourmand; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Ro SSA; Ro 52kD; Ro 60kD; p40phox; Ig classes; systemic lupus erythematosus; Sjögren s syndrome; autoantibodies; immunoblotting;

    Sammanfattning : Autoantibodies to the Ro/SSA and La/SSB antigens are present in sera from patients with Sjögren's syndrome (SS) and systemic lupus erythematosus (SLE). The Ro/SSA autoantigen consists of a 52kD and a 60kD protein, complexed with one of four small RNAs. The La protein can also associate with the complex. LÄS MER

  3. 22. Functional studies of the ubiquitin proteasome system using GFP based reporters

    Författare :Kristina Lindsten; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Ubiquitin; proteasome; green fluorescent protein GFP ; degradation; inhibitor; Gly-Ala repeat; neurodegencration; poly-Gln aggregates.;

    Sammanfattning : Ubiquitin-dependent proteasomal degradation is of paramount importance for cellular processes such as cell cycle progression, transcriptional regulation, apoptosis and disposal of misfolded and aberrant proteins. Moreover the ubiquitin-proteasome system is the main producer of peptides used by major histocompatibility complex class 1 for antigen presentation. LÄS MER

  4. 23. Genetic modification of human natural killer cells and possible applications thereof

    Författare :Kyriakos Konstantinidis; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Natural killer cells; retroviral transduction; interleukin-2; endoplasmic reticulum targeting; green fluorescent protein; multiple myeloma;

    Sammanfattning : Natural killer (NK) cells are dependent on the presence of interleukin-2 (IL-2) for their survival and for their cytotoxicity against tumor cells. Currently, for in vitro expansion of NK cells from peripheral blood mononuclear cells (PBMCs), IL-2 is of great importance. LÄS MER

  5. 24. Selective insulin signaling in the pancreatic beta-cell via the two insulin receptor isoforms

    Författare :Sabine Uhles; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Insulin receptor; signal transduction; gene expression regulation; insulin; glucokinase; cfos; pancreatic beta cell; membrane microdomains; green fluorescent protein.;

    Sammanfattning : Insulin exhibits pleiotropic effects that are tissue- as well as development-dependent. However, the mechanisms by which insulin gains selective effects are poorly understood. Selectivity in insulin signaling is currently discussed as the result of the activation of specific signal transduction pathways. LÄS MER

  7. 25. Regulation of the ubiquitin-proteasome system : characterization of viral and cellular stabilization signals

    Författare :Stijn Heessen; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Epstein-Barr virus EBV ; glycine-alanine GA repeat; green fluorescent protein GFP ; degron; proteasome; Rad23; ubiquitin; ubiquitin- associated UBA domain;

    Sammanfattning : The ubiquitin-proteasome system plays a fundamental role in virtually every cellular process. Degradation of endogenous proteins by this system is the major source for antigenic peptides that are presented to MHC class I-restricted cytotoxic T cells. LÄS MER