Sökning: "green fluorescent protein."

Visar resultat 16 - 20 av 92 avhandlingar innehållade orden green fluorescent protein..

  1. 16. Dynamic protein trafficking of the nuclear membrane and in peroxisomes

    Författare :Charlotta Buch; Einar Hallberg; Howard J. Worman; Södertörns högskola; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : The cell nucleus is enclosed by the nuclear envelope (NE), a double lipid membrane separating the nucleoplasm from the cytoplasm. Transport of macromolecules between the nucleus and the cytoplasm takes places through nuclear pore complexes (NPCs) in a selective and energy dependent manner. LÄS MER

  3. 17. Rational and combinatorial genetic engineering approaches for improved recombinant protein production and purification

    Författare :Nina Bandmann; Per-Åke Nygren; Helena Berglund; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Aqueous two-phase system; combinatorial library; expression vector; flow cytometry; fusion tag; partitioning; peptide library; phage display; recombinant proteins; ribosomal binding site; translation initiation; Bioengineering; Bioteknik;

    Sammanfattning : The bacterium Escherichia coli (E. coli) is in many situations an ideal host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve sufficiently high product quantities. LÄS MER

  4. 18. HOST EPIGENETIC REGULATOR BMI1 AND VIRAL FIBER PROTEIN FOR EQUILIBRIUM BETWEEN ADENOVIRUS AND HOST

    Författare :Manli Na; Neurokirurgi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Adenovirus; BMI1; Dual-gene expression; Epigenetic; Equilibrium; Fiber; HSC; HDAC; TSA;

    Sammanfattning : Human adenoviruses (Ads) are broadly used in cancer gene therapy, vaccine development and gene delivery. The modified Ad5 or Ad2 viruses have been successfully used as oncolytic agents in pre-clinical studies. However they could not be translated into clinic utility due to a number of limitations including inefficient Ad spread among tumor cells. LÄS MER

  5. 19. The ubiquitin-proteasome system during proteotoxic stress

    Författare :Victoria Menéndez Benito; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Ubiquitin; proteasome; green fluorescent protein GFP ; degradation; inhibitor; neurodegeneration; stress;

    Sammanfattning : The dual function of the ubiquitin-proteasome system in protein quality control and as a master regulator of vital cellular processes places the system in a delicate position. This might be particularly relevant under patho-physiological conditions such as endoplasmic reticulum stress, which provoke the accumulation of aberrant proteins. LÄS MER

  7. 20. The role of the ubiquitin-proteasome system in neurodegenerative disorders

    Författare :Lisette Gerridina Gezina Catharina Verhoef; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Degradation; green fluorescent protein GFP ; neurodegeneration; polyglutamine; proteasome; UBB 1; ubiquitin;

    Sammanfattning : Neurodegenerative disorders are a heterogeneous group of clinically and pathologically diverse diseases. The diseases are characterised by selective loss of neurons. in specific regions of the brain. The result is disruption of motor, sensory or cognitive systems, leading to severe disability of the patients. LÄS MER