Sökning: "green fluorescent protein."
Visar resultat 11 - 15 av 92 avhandlingar innehållade orden green fluorescent protein..
11. New preclinical strategies for characterization and development of anticancer drugs
Sammanfattning : Increased understanding of the molecular mechanisms underlying cancer development has shifted drug discovery towards target driven drug development the last decades, but the development of effective cancer drugs has been hampered by the lack of predictive preclinical models. 3-D cultures, considered to more accurately reflect solid tumors in vivo, have been proposed as one way to increase the predictability of clinical efficacy in cancer drug discovery and development. LÄS MER
12. Mechanisms of insulin exocytosis and release
Sammanfattning : Endocrine cells as well as neurons release their hormones and transmitters by regulated exocytosis. In the pancreatic B-cell, stimuli like glucose initiate biochemical and electrical processes that culminate in influx of Ca2+, which then triggers exocytosis of insulin-containing granules. LÄS MER
13. GFP as a tool to monitor membrane protein topology and overexpression in Escherichia coli
Sammanfattning : Membrane proteins are essential for life, and roughly one-quarter of all open reading frames in sequenced genomes code for membrane proteins. Unfortunately, our understanding of membrane proteins lags behind that of soluble proteins, and is best reflected by the fact that only 0. LÄS MER
14. Temporal events in neuronal differentiation and cell death : expression and processing of the Alzheimer's amyloid precursor protein family and a protein at the nuclear pore
Sammanfattning : The present study had two major objectives: 1) to elucidate the involvement of Alzheimer’s amyloid precursor protein (APP) family in neuronal differentiation, and the effect of the Alzheimer’s disease (AD)-linked mutation APPV642I on signal transduction; 2) to investigate the fate of the nuclear pore complex protein POM121 during apoptosis and to examine the possibility of using green fluorescent protein (GFP)-labelled POM121 as a non-invasive sensor of apoptosis in living (non-fixed) cells.APP is the parent protein of the b-amyloid peptide, which is the major peptide constituent in the “senile plaques” of AD. LÄS MER
15. HYDROPHOBIC PEPTIDE TAGS - As tools in bioseparation and investigation of recombinant proteins
Sammanfattning : This thesis describes the possibility of using hydrophobic peptide tags as tools for bioseparation and for the investigation of recombinant proteins. The hydrophobicity of a protein can easily be changed by minor modifications, such as fusions of tyrosine-containing peptides. LÄS MER
