Sökning: "glycosylation."

Visar resultat 1 - 5 av 193 avhandlingar innehållade ordet glycosylation..

1. 1. UDP-sugar metabolizing pyrophosphorylases in plants : formation of precursors for essential glycosylation-reactions

   Författare :Daniel Decker; Leszek A. Kleczkowski; Henrik Vibe Schelle; Umeå universitet; []
   Nyckelord :LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Chemical library screening; Cell wall synthesis; Glycosylation; Nucleotide sugars; Oligomerization; Protein structure; Reverse chemical genetics; Sugar activation; UDP-sugar synthesis; biokemisk farmakologi; biochemical pharmacology; biokemi; Biochemistry; biology; biologi; molekylärbiologi; Molecular Biology;

   Sammanfattning : UDP-sugar metabolizing pyrophosphorylases provide the primary mechanism for de novo synthesis of UDP-sugars, which can then be used for myriads of glycosyltranferase reactions, producing cell wall carbohydrates, sucrose, glycoproteins and glycolipids, as well as many other glycosylated compounds. The pyrophosphorylases can be divided into three families: UDP-Glc pyrophosphorylase (UGPase), UDP-sugar pyrophosphorylase (USPase) and UDP-N-acety lglucosamine pyrophosphorylase (UAGPase), which can be discriminated both by differences in accepted substrate range and amino acid sequences. LÄS MER

3. 2. Bacterial modulation of host glycosylation - in infection, biotechnology, and therapy

   Författare :Jonathan Sjögren; Infektionsmedicin; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; IgG; endoglycosidase; Streptococcus pyogenes; glycosylation; EndoS;

   Sammanfattning : A majority of the proteins of the immune system are glycosylated and the glycans of IgG are essential for its functionality. Bacteria display enzymes that modulate the glycans of the immune system to weaken the host defense and favor bacterial survival. LÄS MER

4. 3. Development and application of alternative methods for profiling proteins N-glycosylation

   Författare :Alessandro Quaranta; Leopold Ilag; Niclas Karlsson; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; N-glycosylation; Glycomics; Glycosylation Biomarkers; Intact Glycoproteins; Glycoform Quantification; Mass Spectrometry; Ion Mobility Spectrometry; MALDI-MS; Paper Spray Ionization; Microfluidics; Magnetic Beads; Immunoaffinity Purification; Nanobodies; Analytical Chemistry; analytisk kemi;

   Sammanfattning : Glycosylation is a post-translational modification (PTM) that exerts profound structural and functional effects on the modified protein. Glycan synthesis and conjugation to proteins are regulated by a myriad of factors, both genetic and environmental, and are also influenced by external stressors. LÄS MER

5. 4. Ligation-mediated Molecular Analysis of Influenza Subtypes, Splicing and Protein Glycosylation

   Författare :Tim Conze; Ulf Landegren; Mats Nilsson; Ola Söderberg; Jörn Glökler; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ligase; proximity ligation; gastric cancer; glycosylation; alternative splicing; avian influenza; padlock probe; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Molekylär medicin; Molecular Medicine;

   Sammanfattning : Binder-based assays are employed throughout the life sciences. Powerful signal amplification techniques have enabled detection of very rare molecule species diluted in simple buffers. Unspecific binding of primary binders leads to increased background in more complex samples. LÄS MER

7. 5. Development of mathematical modelling for the glycosylation of IgG in CHO cell cultures

   Författare :Liang Zhang; Véronique Chotteau; Michael J. Betenbaugh; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Chinese hamster ovary cells; glycosylation; IgG; mathematical modelling; experimental design; perfusion; carbon sources; GReBA; Bayesian network; probabilistic graphic model; Bioteknologi; Biotechnology;

   Sammanfattning : Chinese hamster ovary (CHO) cells are the most popular expression system for the production of biopharmaceuticals. More than 80% of the approved monoclonal antibodies (mAbs) or immunoglobulin G (IgG) are produced with these cells. Glycosylation is a usual post- translational modification important for therapeutic mAbs. LÄS MER