Sökning: "glycosidase"

Visar resultat 1 - 5 av 10 avhandlingar innehållade ordet glycosidase.

  1. 1. Structural Studies of Three Glycosidases

    Författare :Anna Larsson; Alwyn Jones; Janet Smith; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Cell and molecular biology; glycosidase; dextranase; mannanase; endoglucanase; SeMet; MAD; crystal structure; Cell- och molekylärbiologi; Cell and molecular biology; Cell- och molekylärbiologi;

    Sammanfattning : Glycosidases hydrolyse the glycosidic bond in carbohydrates. Structural studies of three glycosidases with different substrate specificities are presented in this work.Dextranase catalyzes the hydrolysis of α-1,6-glycosidic linkage in dextran polymers. LÄS MER

  3. 2. Genetic variations in mouse glycosidase activities related to androgenic effects

    Författare :Eva Håkansson; Uppsala universitet; []
    Nyckelord :MEDICINE; MEDICIN;

    Sammanfattning : .... LÄS MER

  4. 3. Exploring beta-mannan acting glycosidase families in hydrolysis and mannosyl transfer

    Författare :Simon Birgersson; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; glycoside hydrolases; beta-mannanase; Transglycosylation; enzymatic synthesis; Enzyme synergy; enzyme engineering; MALDI-TOF; HPLC; NMR;

    Sammanfattning : The thesis revolves around the study of glycoside hydrolases (GH), specifically GH5 β-mannanases and GH36 α-galactosidase (I-III), as well as a GH130 mannoside phosphorylase (I, IV). These enzymes are investigated for their actions on and conversion of hemicellulosic β-mannan, including food-grade galactomannan and softwood galactoglucomannan, along with manno-oligosaccharides (MOS) derived from such polymers. LÄS MER

  5. 4. Beta-Glycosidases from Hyperthermophiles as Biocatalysts

    Författare :Therese Hansson; Avdelningen för klinisk kemi och farmakologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Bioteknik; Biochemical technology; Biokemisk teknik; Proteins; enzymology; Proteiner; enzymologi; Biotechnology; high temperatures; water activity; activity; selectivity; hexanol; hexyl-b-glycoside; galacto-oligosaccharides; lactose; hyperthermostable b-glycosidase; hydrolysis; optimisation; transglycosylation;

    Sammanfattning : The thesis concerns glycosyl transfer with use of b-glycosidases, most of them from hyperthermophilic organisms. Both optimisation of the reaction conditions and protein engineering in terms of site-directed mutagenesis were used to improve b-glycosidase-catalysed reactions. LÄS MER

  7. 5. Synthesis of Carbohydrate Mimics and Development of a Carbohydrate Epimerisation Method

    Författare :Clinton Ramstadius; Ian Cumpstey; Robert Madsen; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Carbohydrate Mimic; Ring-closing Metathesis; Carbohydrate Chemistry; Carbasugar; Aminocarbasugar; Pseudodisaccharide; Glycosidase and Lectin Inhibitors; Organic chemistry; Organisk kemi; organisk kemi; Organic Chemistry;

    Sammanfattning : In this thesis the synthesis of several hydrolytically stable carbohydrate mimics with the potential to function as glycosidase or lectin inhibitors are described. This work is presented in Chapters 2-5. LÄS MER