Sökning: "genotyping technology"

Visar resultat 1 - 5 av 44 avhandlingar innehållade orden genotyping technology.

  1. 1. Genetic Sequence Analysis by Microarray Technology

    Detta är en avhandling från Stockholm : KTH

    Författare :Emilie Hultin; KTH.; [2007]
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Genotyping; single nucleotide polymorphism SNP ; protease-mediated allele-specific extension PrASE ; microarray; tag-array; competitive hybridization; human papillomavirus HPV ; single cell; loss of alleles; differentiation; epidermis.; TECHNOLOGY Bioengineering; TEKNIKVETENSKAP Bioteknik;

    Sammanfattning : Developments within the field of genetic analysis have during the last decade become enormous. Advances in DNA sequencing technology have increased throughput from a thousand bases to over a billion bases in a day and decreased the cost thousandfold per base. LÄS MER

  2. 2. Microarray Technology for Genotyping in Pharmacogenetics

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Ulrika Liljedahl; Uppsala universitet.; [2004]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; microarray; genotyping; pharmacogenetics; molecular medicine; single nucleotide polymorphism; hypertension; Molekylärmedicin; MEDICINE Dermatology and venerology; clinical genetics; internal medicine Internal medicine Molecular medicine genetics and pathology ; MEDICIN Dermatologi och venerologi; klinisk genetik; invärtesmedicin Invärtesmedicin Molekylär medicin genetik och patologi ;

    Sammanfattning : The studies in this thesis describe the development of a microarray based minisequencing system and its application to highly parallel genotyping of single nucleotide polymorphisms. The technical developments included identification of a three-dimensional microarray surface coating with high binding capacity for oligonucleotides modified with amino groups as the most optimal one for the system. LÄS MER

  3. 3. Detection and Characterisation of Salmonella in Animal Feed Samples by PCR-Based Methods

    Detta är en avhandling från Applied Microbiology (LTH)

    Författare :Charlotta Löfström; Lund University.; Lunds universitet.; [2005]
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Food and drink technology; Livsmedelsteknik; Biotechnology; Bioteknik; Biokemisk teknik; Biochemical technology; Analytisk kemi; Analytical chemistry; mykologi; virologi; bakteriologi; Mikrobiologi; validation; Microbiology; bacteriology; virology; mycology; Salmonella; ribotyping; detection; animal feed; randomly amplified polymorphic DNA; pulsed-field gel electrophorsis; pre-PCR processing; PCR; polymerase chain reaction; genotyping;

    Sammanfattning : Animal feed is a recognised source of Salmonella enterica for farm livestock and may also indirectly cause infection in people consuming foods of animal origin. It is therefore important to have rapid, reproducible and specific methods for the detection of Salmonella in feed, and for the characterisation of strains for further epidemiological investigations or to trace the source of contamination in a production facility. LÄS MER

  4. 4. Interrogation of Nucleic Acids by Parallel Threading

    Detta är en avhandling från Stockholm : KTH

    Författare :Erik Pettersson; KTH.; [2007]
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; genotyping; multiplex amplification; trinucleotide threading; single nucleotide polymorphism; genotype calling; Expectation-Maximization; protein-epitope signature tag; expression profiling; Human Protein Atlas; pooled genomic DNA; Pyrosequencing; 454; allelotyping; association studies; bioinformatics; TECHNOLOGY Bioengineering; TEKNIKVETENSKAP Bioteknik;

    Sammanfattning : Advancements in the field of biotechnology are expanding the scientific horizon and a promising era is envisioned with personalized medicine for improved health. The amount of genetic data is growing at an ever-escalating pace due to the availability of novel technologies that allow massively parallel sequencing and whole-genome genotyping, that are supported by the advancements in computer science and information technologies. LÄS MER

  5. 5. Arrayed identification of DNA signatures

    Detta är en avhandling från Stockholm : KTH

    Författare :Max Käller; KTH.; [2005]
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; apyrase; allele-specific extension; competitive hybridization; DNA sequencing; genotyping; human papillomavirus HPV ; MC1R; microarray; mutation; p53; protease; TECHNOLOGY Bioengineering; TEKNIKVETENSKAP Bioteknik;

    Sammanfattning : In this thesis techniques are presented that aim to determine individual DNA signatures by controlled synthesis of nucleic acid multimers. Allele-specific extension reactions with an improved specificity were applied for several genomic purposes. Since DNA polymerases extend some mismatched 3’-end primers, an improved specificity is a concern. LÄS MER