Sökning: "genotyping technology"

Visar resultat 1 - 5 av 38 avhandlingar innehållade orden genotyping technology.

1. 1. Microarray Technology for Genotyping in Pharmacogenetics

   Författare :Ulrika Liljedahl; Ann-Christine Syvänen; Denis Grant; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; microarray; genotyping; pharmacogenetics; molecular medicine; single nucleotide polymorphism; hypertension; Molekylärmedicin; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ;

   Sammanfattning : The studies in this thesis describe the development of a microarray based minisequencing system and its application to highly parallel genotyping of single nucleotide polymorphisms. The technical developments included identification of a three-dimensional microarray surface coating with high binding capacity for oligonucleotides modified with amino groups as the most optimal one for the system. LÄS MER

3. 2. Genetic Sequence Analysis by Microarray Technology

   Författare :Emilie Hultin; Joakim Lundeberg; Afshin Ahmadian; Marie Allen; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Genotyping; single nucleotide polymorphism SNP ; protease-mediated allele-specific extension PrASE ; microarray; tag-array; competitive hybridization; human papillomavirus HPV ; single cell; loss of alleles; differentiation; epidermis.; Bioengineering; Bioteknik;

   Sammanfattning : Developments within the field of genetic analysis have during the last decade become enormous. Advances in DNA sequencing technology have increased throughput from a thousand bases to over a billion bases in a day and decreased the cost thousandfold per base. LÄS MER

4. 3. Detection and Characterisation of Salmonella in Animal Feed Samples by PCR-Based Methods

   Författare :Charlotta Löfström; Teknisk mikrobiologi; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Food and drink technology; Livsmedelsteknik; Biotechnology; Bioteknik; Biokemisk teknik; Biochemical technology; Analytisk kemi; Analytical chemistry; mykologi; virologi; bakteriologi; Mikrobiologi; validation; Microbiology; bacteriology; virology; mycology; Salmonella; ribotyping; detection; animal feed; randomly amplified polymorphic DNA; pulsed-field gel electrophorsis; pre-PCR processing; PCR; polymerase chain reaction; genotyping;

   Sammanfattning : Animal feed is a recognised source of Salmonella enterica for farm livestock and may also indirectly cause infection in people consuming foods of animal origin. It is therefore important to have rapid, reproducible and specific methods for the detection of Salmonella in feed, and for the characterisation of strains for further epidemiological investigations or to trace the source of contamination in a production facility. LÄS MER

5. 4. Analysis of Complex Genetic Traits in Population Cohorts using High-throughput Genotyping Technology

   Författare :Andreas Dahlgren; Ann-Christine Syvänen; Håkan Melhus; Markus Perola; Struan Grant; Uppsala universitet; []
   Nyckelord :Molecular medicine; SNP; TCF7L2; HHEX; COL11A1; ESR1; body height; type 2 diabetes mellitus; proinsulin; ULSAM; complex genetic trait; genotyping technology; Molekylärmedicin;

   Sammanfattning : Most human traits and common diseases have a complex genetic makeup involving more than one gene. The work presented in this thesis investigates standing body height and the common disease type 2 diabetes mellitus (T2DM). LÄS MER

7. 5. Interrogation of Nucleic Acids by Parallel Threading

   Författare :Erik Pettersson; Joakim Lundeberg; Afshin Ahmadian; Ann-Christine Syvänen; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; genotyping; multiplex amplification; trinucleotide threading; single nucleotide polymorphism; genotype calling; Expectation-Maximization; protein-epitope signature tag; expression profiling; Human Protein Atlas; pooled genomic DNA; Pyrosequencing; 454; allelotyping; association studies; bioinformatics; Bioengineering; Bioteknik;

   Sammanfattning : Advancements in the field of biotechnology are expanding the scientific horizon and a promising era is envisioned with personalized medicine for improved health. The amount of genetic data is growing at an ever-escalating pace due to the availability of novel technologies that allow massively parallel sequencing and whole-genome genotyping, that are supported by the advancements in computer science and information technologies. LÄS MER