Sökning: "gen larsson"

Visar resultat 1 - 5 av 14 avhandlingar innehållade orden gen larsson.

  1. 1. Biobased production of succinic acid by Escherichia coli fermentation

    Författare :Christian Andersson; Gen Larsson; Luleå tekniska universitet; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biokemisk processteknik; Biochemical Process Engineering;

    Sammanfattning : The prospects of peak oil, climate change and the dependency of fossil carbon have urged research and development of production methods for the manufacture of fuels and chemicals from renewable resources (biomass). The present thesis illustrates different aspects of biobased succinic acid production by a metabolically engineered E. coli strain. LÄS MER

  3. 2. Pharmaceuticals – improved removal from municipal wastewater and their occurrence in the Baltic Sea

    Författare :Berndt Björlenius; Gen Larsson; Antonius J. A. van Maris; Hallvard Ødegaard; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Advanced wastewater treatment; WWTP; pilot plant; pharmaceutical residues; removal of pharmaceuticals; activated carbon; ozonation; nanofiltration; biomarker; Baltic Sea; Bioteknologi; Biotechnology;

    Sammanfattning : Pharmaceutical residues are found in the environment due to extensive use in human and veterinary medicine. The active pharmaceutical ingredients (APIs) have a potential impact in non-target organisms. Municipal wastewater treatment plants (WWTPs) are not designed to remove APIs. LÄS MER

  4. 3. Growth rate control of periplasmic product retention in Escherichia coli

    Författare :Emma Bäcklund; Gen Larsson; Stefan Schmidt; Amelie Eriksson Karlström; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Escherichia coli; fed-batch; outer membrane proteins; recombinant proteins; specific growth rate; periplasmic retention; phosphotransferase system; high cell density cultivation; acetate formation; Bioengineering; Bioteknik;

    Sammanfattning : The recombinant product is secreted to the periplasm in many processes where E. coli is used as host. One drawback with secretion is the undesired leakage of the periplasmic products to the medium. The aim of this work was to find strategies to influence the periplasmic retention of recombinant products. LÄS MER

  5. 4. Impact of glucose uptake rate on recombinant protein production in Escherichia coli

    Författare :Emma Bäcklund; Gen Larsson; Olle Holst; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; AIDA-autotransporter; Escherichia coli; fed-batch; glucose uptake rate; integral membrane proteins; outer membrane proteins; periplasmic retention; phosphotransferase system; recombinant proteins; specific growth rate; surface expression; Biology; Biologi;

    Sammanfattning : Escherichia coli (E. coli) is an attractive host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve high product quantities. LÄS MER

  7. 5. Strain- and bioprocess-design strategies to increase production of (R)-3-hydroxybutyrate by Escherichia coli

    Författare :Mónica Guevara-Martínez; Gen Larsson; Antonius J. A. van Maris; Pau Ferrer; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; E. coli; R -3-hydroxybutyrate; metabolic engineering; bioprocess design; NADPH; acetic acid; thioesterase; BAC; Bioteknologi; Biotechnology;

    Sammanfattning : Microbial bio-based processes have emerged as an alternative to replace fossil-based processes for the production of fuels and chemicals. (R)-3-hydroxybutyrate (3HB) is a medium-value chemical that has gained special attention as a precursor of antibiotics and vitamins, as a monomer for the synthesis of tailor-made polyesters and as a nutritional source for eukaryotic cells. LÄS MER