Sökning: "fusion protein"
Visar resultat 6 - 10 av 338 avhandlingar innehållade orden fusion protein.
6. Strategies for facilitated protein recovery after recombinant production in Escherichia coli
Sammanfattning : The successful genomic era has resulted in a great demand for efficient production and purification of proteins. The main objective of the work described in this thesis was to develop methods to facilitate recovery of target proteins after recombinant production in Escherichia coli. LÄS MER
7. Metal Affinity Peptides in Biotechnological Applications As tools in protein purification, protein stabilisation, metal quantification and metal tolerance of bacteria and plants
Sammanfattning : Metal affinity tags have been fused to proteins and demonstrated to function as tools in protein purification, protein stabilisation, quantitative metal analysis and metal tolerance of bacteria and plants. In biotechnological applications proteins frequently have to function in nonnatural environments with harsh conditions which impose requirements for high stability on the proteins. LÄS MER
8. Gene fusion in protein engineering : design of novel peptides and bifunctional enzymes
Sammanfattning : The gene fusion technique is widely used in protein engineering and in this thesis it constitutes the basis of both the construction of artificial bifunctional enzymes and the display of two novel peptides. Artificial bifunctional enzymes have been used as model systems in the studies of native multifunctional enzymes and proximity effects within these complexes. LÄS MER
9. Advanced Fluorescence Microscopy to Study Plasma Membrane Protein Dynamics
Sammanfattning : Membrane protein dynamics is of great importance for living organisms. The precise localization of proteins composing a synapse on the membrane facing a nerve terminus is essential for proper functioning of the nervous system. In muscle fibers, the nicotinic acetylcholine is densely packed under the motor nerve termini. LÄS MER
10. On bacterial formats in protein library technology
Sammanfattning : Millions of years of evolution have resulted in an immense number of different proteins, which participate in virtually every process within cells and thus are of utmost importance for allknown forms of life. In addition, there are several examples of natural proteins which have found use in applications outside their natural environment, such as the use of enzymes infood industry and washing powders or the use of antibodies in diagnostic, bioseparation or therapeutic applications. LÄS MER