Sökning: "fusion protein"

Visar resultat 21 - 25 av 338 avhandlingar innehållade orden fusion protein.

  1. 21. Protein production and purification in structural genomics

    Författare :Martin Hammarström; Per-Åke Nygren; Torleif Härd; Helena Berglund; Herman van Tilbeurgh; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Recombinational cloning; Escherichia coli; gene expression; fusion proteins; solubility; circular dichroism; nuclear magnetic resonance; Structural biochemistry; Strukturbiokemi;

    Sammanfattning : The number of gene products available for structural and functional study is increasing at an unprecedented rate as a result of the successful whole genome sequencing projects. Systematic structure determination of proteins on a genomic scale, called structural genomics, can significantly contribute to the field of protein science and to functional annotation of newly identified genes. LÄS MER

  3. 22. Molecular Mechanisms of the DEK Protein in Leukemia

    Författare :Carl Sandén; Avdelningen för hematologi och transfusionsmedicin; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; DEK; DEK-NUP214; fusion gene; acute myeloid leukemia; proliferation; mTOR; ChIP-seq;

    Sammanfattning : The DEK gene is upregulated in a growing number of cancers and translocated to form the DEK-NUP214 fusion gene in a subset of acute myeloid leukemia. In this thesis, we studied the cellular and molecular functions of the DEK and DEK-NUP214 proteins. LÄS MER

  4. 23. Protein interactions involved in viral replication characterized by T7 phage display

    Författare :Hamid Houshmand; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; protein-protein interaktion; fagdisplayteknik; HCV; polyomavirus; Biokemi; Biochemistry; Biokemi; Medical Virology; medicinsk virologi;

    Sammanfattning : Protein interactions are essential for many cellular processes. By utilizing protein-protein interactions, viruses can infect their host cells and replicate. Phage display can be used for identification and characterization of protein-protein interactions. LÄS MER

  5. 24. Hydrophobic Fusion Tags: Implications For Bioseparation and Cellular Expression

    Författare :Anna Collén; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein production; secretion; aqueous two-phase systems; Trichoderma reesei; endoglucanase; Biochemistry; Metabolism; Biokemi; metabolism;

    Sammanfattning : The studies in this thesis have shown that the partitioning of endoglucanase I (EGI, Cel7B) from T. reesei could be significantly improved by relatively minor genetic engineering. By adding short peptides composed of tryptophan and proline residues to EGI, extreme partitioning could be obtained. LÄS MER

  7. 25. Bax and oxidized phospholipids - a deadly complex : Apoptotic protein-lipid assemblies studied by MAS NMR spectroscopy

    Författare :Artur Dingeldein; Gerhard Gröbner; Mikael Elofsson; Tobias Sparrman; Daniel Topgaard; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NMR spectroscopy; mitochondrial membranes; apoptosis; membrane dynamics and organization; Bcl-2 family proteins; Bax protein; biokemi; Biochemistry;

    Sammanfattning : Mitochondria are renowned for their vital role as cellular powerhouses because they provide ATP via cellular respiration. Additionally, these organelles also play an important role in other physiological processes, such as apoptosis. LÄS MER