Sökning: "fluorescence in-situ hybridization"

Visar resultat 11 - 15 av 57 avhandlingar innehållade orden fluorescence in-situ hybridization.

1. 11. Fluorescence in situ hybridization for the detection of genetic alterations in prostate and bladder malignancies : significance as a genetic marker to predict the patient prognosis

   Författare :Hideyasu Matsuyama; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :prostate cancer bladder cancer tumor suppressor gene chromosomal deletion chromosome 8p; chromosome lOq chromosome 16q; fluorescence in situ hybridization Prostate cancer and bladder cancer are common urological malignancies in man; while a lack of;

   Sammanfattning : Prostate cancer and bladder cancer are common urological malignancies in man, while a lack of basic information underlying the oncogenesis and the tumor progression makes it difficult to form therapeutic strategies against these malignancies. Recent advances of molecular biology revealed that genetic alteration play a crucial role in the oncogenesis and progression of these tumors. LÄS MER

3. 12. Arrayed identification of DNA signatures

   Författare :Max Käller; Lundeberg Joakim; Mats Nilsson; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; apyrase; allele-specific extension; competitive hybridization; DNA sequencing; genotyping; human papillomavirus HPV ; MC1R; microarray; mutation; p53; protease; Bioengineering; Bioteknik;

   Sammanfattning : In this thesis techniques are presented that aim to determine individual DNA signatures by controlled synthesis of nucleic acid multimers. Allele-specific extension reactions with an improved specificity were applied for several genomic purposes. Since DNA polymerases extend some mismatched 3’-end primers, an improved specificity is a concern. LÄS MER

4. 13. Hypothalamic neuronal circuits involved in the regulation of food intake and body weight : histochemical studies in lean rats and obese mutant mice

   Författare :Matilda Bäckberg; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :AGRP; arcuate nucleus; BBB; CART; GABA; GABA receptors; immunohistochemistry; in situ hybridization; hypothalamus; LHA; leptin; MCH; NPY; orexin; POMC; tubby;

   Sammanfattning : Obesity is a major health problem because of its dramatic increase in prevalence and its association with type 2 diabetes, coronary heart diseases, hypertension and stroke. It is well known that signals generated in response to feeding behavior are integrated in the brain. LÄS MER

5. 14. Molecular markers reflecting malignant transformation and tumor progression

   Författare :Patricia Stoltzfus; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :Molecular markers; interphase fluorescence in situ hytidization; genetic diagnostic test; laminin-5 gamma2 chain; immunohistochemistry; precancerous lesions; invasiveness; breast cancer; cervical squamous cell carcinoma; tongue carcinoma; vulvar carcinoma;

   Sammanfattning : The early detection of neoplastic lesions is most critical in the successful treatment of malignant disease. This thesis investigates molecular targets for an improved detection and diagnosis of cancer by exploring the value of interphase fluorescence in situ hybridization (FISH) to detect specific chromosomal aneuploidies and gene amplifications, and laminin-5 gamma2 chain expression detected by immunohistochemistry as a marker for invasiveness. LÄS MER

7. 15. Technology development for genome and polymorphism analysis

   Författare :Magnus Jobs; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :SNP; Genotyping technology; DASH; Array technology; Assay design;

   Sammanfattning : Single nucleotide polymorphism (SNP) is widely expected to provide a multitude of benefits in areas of medical research. There is, however, a growing disparity between the rate of SNP discovery and the development of effective technologies for large scale SNP scoring. LÄS MER