Sökning: "fluorescence correlation spectroscopy FCS"

Visar resultat 1 - 5 av 24 avhandlingar innehållade orden fluorescence correlation spectroscopy FCS.

  1. 1. Temporal Modulation in Fluorescence Spectroscopy and Imaging for Biological Applications

    Författare :Gustav Persson; Jerker Widengren; Jörg Enderlein; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; fluorescence; spectroscopy; microscopy; modulated excitation; intensity modulation; fluorescence correlation spectroscopy; transient states; molecular kinetics; Physics; Fysik;

    Sammanfattning : This thesis explores the benefits of intensity modulation for the purpose of extending the range of applications of fluorescence spectroscopy and imaging in cellular and molecular biology and medicine. Long-lived transient states of fluorescent molecules can, because of their long lifetimes, be used to detect subtle changes in the microenvironment of the molecule. LÄS MER

  3. 2. Time-Varying Excitation in Fluorescence Spectroscopy for Biological Applications

    Författare :Gustav Persson; Jerker Widengren; Måns Ehrenberg; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; fluorescence; spectroscopy; molecular kinetics; Physics; Fysik;

    Sammanfattning : The focus of this thesis is to explore and use the benefits of time-varying excitation in fluorescence spectroscopy for studies of biomolecular dynamics. Two new techniques taking advantage of modulated excitation are presented. LÄS MER

  4. 3. Laser Scanning Multiphoton Microscopy – Focusing on Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Imaging for Biomedical Applications

    Författare :Jeemol James; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Laser scanning multiphoton microscopy; intrinsic cellular fluorophores.; fluorescence correlation spectroscopy; fluorescence lifetime imaging; melanoma metastasis;

    Sammanfattning : Laser scanning multiphoton microscopy (MPM) is considered as a non-invasive technology for three-dimensional imaging of complex biological tissues. The quantitative potential of the MPM is not investigated as much as qualitative imaging. LÄS MER

  5. 4. Fluorescence correlation spectroscopy, photophysical aspects and applications

    Författare :Jerker Widengren; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Fluorescence correlation spectroscopy (FCS) is a technique where dynamic processes on the molecular level are studied by the use of fluorescence. The molecules are excited within a focused stationary laser beam and the resulting fluorescence fluctuations are analyzed in the form of an autocorrelation function. LÄS MER

  7. 5. Development and application of ultra-sensitive fluorescence spectroscopy and microscopy for biomolecular interaction studies

    Författare :Lei Xu; Jerker Widengren; Gerhard Schütz; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; single molecule spectroscopy; fluorescence correlation spectroscopy; stimulated emission microscopy; cancer; biomolecular interaction; co-localization; Biological Physics; Biologisk fysik;

    Sammanfattning : This thesis describes the development of sensitive and high-resolution fluorescence spectroscopic and microscopic techniques and their application to probe biomolecules and their interactions in solution, lipid membrane model systems and in cells. Paper I-IV are largely focused on methodological developments. LÄS MER