Sökning: "fluorescence contrast"
Visar resultat 21 - 25 av 104 avhandlingar innehållade orden fluorescence contrast.
21. Groove-binding Unsymmetrical Cyanine Dyes for DNA Applications
Sammanfattning : This work focuses on interactions between DNA and a group of newly developed unsymmetrical cyanine dyes. The aim of the work was to discover fluorescent dyes that can be used in biophysical studies of natural DNA where minimum perturbation of the DNA-helix is essential, where a slow dissociation rate of the dyes is needed and where the ionic strength is an important parameter. LÄS MER
22. Structural and physicochemical studies on metal and porphyrin binding by the Bacillus subtilis ferrochelatase
Sammanfattning : The enzyme ferrochelatase (EC 4.99.1.1) is the terminal enzyme in the biosynthetic pathway of heme b and catalyzes the insertion of Fe(II) into protoporphyrin IX. LÄS MER
23. Self-assembly of amyloid-β peptides in the presence of metal ions and interacting molecules – a detour of amyloid building blocks
Sammanfattning : Misfolding of proteins into amyloid structures is implicated as a pathological feature in several neurodegenerative diseases and the molecular causes are still unclear. One typical characteristic of Alzheimer’s disease is self-assembly and accumulation of soluble amyloid-β (Aβ) peptides into insoluble fibrils and plaques. LÄS MER
24. Multi-dimensional imaging : A tool to unravel material properties at the nanoscale
Sammanfattning : Optical microscopy has revolutionized the way we understand our world and has become a crucial tool in a range of disciplines such as biology, physics, chemistry, and material sciences. The development of new microscopy techniques has been capital to its success and wide applicability. LÄS MER
25. Concentration measurements in single particle microscopy
Sammanfattning : The topic of this thesis is the introduction of two novel methods for using single particle microscopy as a tool for absolute number concentration measurements of Brownian particles. The key idea of both methods is that in order to estimate number concentration, the size of the (three-dimensional) particle detection region has to be estimated. LÄS MER