Sökning: "fluorescence contrast"

Visar resultat 21 - 25 av 104 avhandlingar innehållade orden fluorescence contrast.

  1. 21. Groove-binding Unsymmetrical Cyanine Dyes for DNA Applications

    Författare :Maja Eriksson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; polarized spectroscopy; bacteriophage T5; cryo-transmission electron microscopy; dynamic light scattering; DNA; intercalation; cyanine dyes; groove-binding; fluorescence spectroscopy; gel electrophoresis;

    Sammanfattning : This work focuses on interactions between DNA and a group of newly developed unsymmetrical cyanine dyes. The aim of the work was to discover fluorescent dyes that can be used in biophysical studies of natural DNA where minimum perturbation of the DNA-helix is essential, where a slow dissociation rate of the dyes is needed and where the ionic strength is an important parameter. LÄS MER

  3. 22. Structural and physicochemical studies on metal and porphyrin binding by the Bacillus subtilis ferrochelatase

    Författare :Mattias Hansson; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; metal specificity; metal binding; porphyrin metallation; time resolved crystallography; fluorescence quenching; X-ray structure; inclusion bodies; Bacillus subtilis; heme biosynthesis; ferrochelatase; hemH;

    Sammanfattning : The enzyme ferrochelatase (EC 4.99.1.1) is the terminal enzyme in the biosynthetic pathway of heme b and catalyzes the insertion of Fe(II) into protoporphyrin IX. LÄS MER

  4. 23. Self-assembly of amyloid-β peptides in the presence of metal ions and interacting molecules – a detour of amyloid building blocks

    Författare :Cecilia Mörman; Astrid Gräslund; Daniel Otzen; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; biophysics; Alzheimer’s disease; protein aggregation; amyloid formation; amyloid-β peptide; aggregation kinetics; interactions; metal ions; designed peptide constructs; Tau protein; NMR; circular dichroism; fluorescence spectroscopy; Biophysics; biofysik;

    Sammanfattning : Misfolding of proteins into amyloid structures is implicated as a pathological feature in several neurodegenerative diseases and the molecular causes are still unclear. One typical characteristic of Alzheimer’s disease is self-assembly and accumulation of soluble amyloid-β (Aβ) peptides into insoluble fibrils and plaques. LÄS MER

  5. 24. Multi-dimensional imaging : A tool to unravel material properties at the nanoscale

    Författare :Boris Louis; NanoLund: Centre for Nanoscience; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Optical microscopy; Fluorescence Microscopy; Multi-dimensional imaging; Material sciences; Optical Trapping; Single-molecule imaging and tracking; Single-molecule spectroscopy;

    Sammanfattning : Optical microscopy has revolutionized the way we understand our world and has become a crucial tool in a range of disciplines such as biology, physics, chemistry, and material sciences. The development of new microscopy techniques has been capital to its success and wide applicability. LÄS MER

  7. 25. Concentration measurements in single particle microscopy

    Författare :Magnus Röding; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; diffusion; fluorescence microscopy; nanoparticle characterization; number concentration; optical wide-field microscopy; single particle tracking; Smoluchowski process; diffusion;

    Sammanfattning : The topic of this thesis is the introduction of two novel methods for using single particle microscopy as a tool for absolute number concentration measurements of Brownian particles. The key idea of both methods is that in order to estimate number concentration, the size of the (three-dimensional) particle detection region has to be estimated. LÄS MER