Sökning: "firefly luciferase"

Visar resultat 1 - 5 av 9 avhandlingar innehållade orden firefly luciferase.

  1. 1. Advancements in Firefly Luciferase-Based Assays and Pyrosequencing Technology

    Författare :Jonas Eriksson; KTH; []
    Nyckelord :bioluminescence; osmolytes; glycine betaine; thermostability; firefly luciferase; inorganic pyrophosphatase; inorganic pyrophosphate; Pyrosequencing technology; secondary DNA-structures; Sequenase; Klenow-polymerase; reaction rates; temperature;

    Sammanfattning : Pyrosequencing is a new DNA sequencing method relying on thesequencing-by-synthesis principle and bioluminometric detectionof nucleotide incorporation events. The objective of thisthesis was improvement of the Pyrosequencing method byincreasing the thermal stability of firefly luciferase, and byintroducing an alternative DNA polymerase and a new nucleotideanalog. LÄS MER

  3. 2. Photochemical properties of phytochrome and firefly luciferase chromophores: A theoretical study

    Författare :Olle Falklöf; Bo Durbeej; Patrick Norman; Marcus Lundberg; Linköpings universitet; []
    Nyckelord :;

    Sammanfattning : This licentiate thesis presents computational chemistry studies on photochemical properties of phytochrome and firefly luciferase chromophores.Phytochromes are bilin-containing proteins that based on the ambient light environment regulate a number of physiological and developmental processes in bacteria, cyanobacteria, fungi and plants. LÄS MER

  4. 3. Development of bioluminescent reagents for kinetic analysis and some applications in clinical chemistry

    Författare :Inger Styrélius; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : Firefly luciferase and a bacterial luciferase system have been used to develop analytical methods suitable for use in clinical analysis.The influence of the level of NADH:FMN oxidoreductase on the kinetics of light emission in the bacterial luciferase system was studied. LÄS MER

  5. 4. Quantitative monitoring of microorganisms in environmental samples using biomarkers

    Författare :Annelie Elväng; James Prosser; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; biokemi; Biochemistry;

    Sammanfattning : The use of microorganisms in environmental biotechnology is an area of growing interest. Microorganisms have been used for diverse applications, such as biological fertilizers, biological pesticides and bioremediation of toxic chemicals. In many cases, the bacteria of interest have been genetically alterred to improve certain traits. LÄS MER

  7. 5. Pyrosequencing

    Författare :Samer Karamohamed; KTH; []
    Nyckelord :;

    Sammanfattning : Pyrosequencing is a method that can be used in almost allDNA sequencing applications. The method uses coupled reactionsof ATP sulfurylase and firefly luciferase to monitor theinorganic pyrophosphate (PPi) released during nucleotideincorporation onto a DNA primer. LÄS MER