Sökning: "fed-batch culture"

Visar resultat 1 - 5 av 16 avhandlingar innehållade orden fed-batch culture.

  1. 1. Development of a protein-free fed-batch process for NS0 cells: studies on regulation of proliferation

    Författare :Erika Spens; Lena Häggström; Jean-Louis Goergen; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NS0 myeloma cells; protein-free medium; fed-batch; conditioned medium; autocrine growth factors; abortive proliferation; protease activity; aprotinin; gp130; Bioengineering; Bioteknik;

    Sammanfattning : The overall objective of this study was to investigate how NS0 cell proliferation is regulated in protein-free media. The hypothesis was that during the adaptation to growth factor-free media, animal cell lines start to produce their own autocrine growth factors to support proliferation, and after some time in a culture the effects of these factors are lost which results in cessation of proliferation. LÄS MER

  3. 2. The temperature-limited fed-batch technique for control of Escherichia coli cultures

    Författare :Marie Svensson; Sven-Olof Enfors; John Villadsen; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Endotoxin; Escherichia coli; fed-batch technique; glucose-limitation; mathematical model; Bioengineering; Bioteknik;

    Sammanfattning : The objective of this study was to investigate the physiology and productivity in Escherichia colicultures with emphasis on the temperature-limited fed-batch (TLFB) culture. The TLFB techniquecontrols the oxygen consumption rate of the growing culture by a gradually declining temperaturefrom 37-35 °C down to 20-18 °C. LÄS MER

  4. 3. Process techniques for production of recombinant proteins with Picha pastoris

    Författare :Mehmedalija Jahic; KTH; []
    Nyckelord :p. Pastoris; fed-batch culture; proteolysis; modeling; TECHNOLOGY; TEKNIKVETENSKAP;

    Sammanfattning : .... LÄS MER

  5. 4. Control of Proteolysis of Recombinant Proteins in Escherichia coli

    Författare :Aleksei Rozkov; KTH; []
    Nyckelord :proteolysis; recombinant protein; E. coli; bioprocess scale-up; protein A; ATP; sulfite; amino acids; growth inhibition; fed-batch culture; ZZT2;

    Sammanfattning : .... LÄS MER

  7. 5. Physiology of Escherichia coli in batch and fed-batch cultures with special emphasis on amino acid and glucose metabolism

    Författare :Ling Han; KTH; []
    Nyckelord :escherichia coli; amino acids; glycine; quorum sensing; metabolic switch; metabolite po9ols; carbon balance; outer membrne; lipopolysaccharide; batch culture;

    Sammanfattning : The objective of this work is to better understand themetabolism and physiology ofEscherichiacoli(W3110) in defined medium cultures with thelong-term goal of improving cell yield and recombinant proteinproductivity.The order of amino acid utilization inE. colibatch cultures was investigated in a medium with16 amino acids and glucose. LÄS MER