Sökning: "expressed sequence tag"

Visar resultat 1 - 5 av 21 avhandlingar innehållade orden expressed sequence tag.

  1. 1. Sequencing of genes and genomes

    Författare :Fredrik Sterky; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; DNA sequencing; genome analysis; expressed sequence tag; expression analysis; complementary DNA; biotin-capture; solid-phase technology; automation; poplar; Bioengineering; Bioteknik;

    Sammanfattning : .... LÄS MER

  3. 2. Computational approaches for in-depth analysis of cDNA sequence tags

    Författare :Per Unneberg; Joakim Lundberg; Leif Andersson; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; cDNA; EST; gene expression; SNP; SAGE; polymorphism; Bioteknik; Bioengineering; Bioteknik;

    Sammanfattning : Major recent improvements in biotechnology have led to an accelerated production of DNA sequences. The completion of the human genome sequence, along with the genomes of more than two hundred other species, has marked the arrival of the genome era. The ultimate goal is to understand the structure and function of genomes and their genes. LÄS MER

  4. 3. Molecular Phylogenetics of Mammals

    Författare :Morgan Kullberg; Biologiska institutionen; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Genetics; EST; divergence times; phylogenomics; phylogenetic reconstruction; Mammals; Eutheria; cytogenetics; Genetik; cytogenetik;

    Sammanfattning : In this thesis, the phylogenetic relationships of the Mammalia have been studied at various levels. Different sources of genetic information have been evaluated and used as phylogenetic markers. These include the well-known mitochondrial genome, cDNA from housekeeping genes and expressed sequence tags from nuclear genes. LÄS MER

  5. 4. Mining the transcriptome - methods and applications

    Författare :Valtteri Wirta; Joakim Lundeberg; John Quackenbush; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; transcriptome; gene expression profiling; EST; microarray; RNA amplification; stem cells; neurosphere; Genetic engineering including functional genomics; Genteknik inkl. funktionsgenomik;

    Sammanfattning : Regulation of gene expression occupies a central role in the control of the flow of genetic information from genes to proteins. Regulatory events on multiple levels ensure that the majority of the genes are expressed under controlled circumstances to yield temporally controlled, cell and tissue-specific expression patterns. LÄS MER

  7. 5. Engineering of Affibody molecules for Radionuclide Molecular Imaging and Intracellular Targeting

    Författare :Camilla Hofström; Torbjörn Gräslund; Serge Muyldermans; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Affibody molecules; affinity proteins; radionuclide molecular imaging; intracellular targeting; EGFR; HER2; IGF-1R; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

    Sammanfattning : Affibody molecules are small (7 kDa) affinity proteins of non-immunoglobulin origin that have been generated to specifically interact with a large number of clinically important molecular targets.In this thesis, Affibody molecules have been employed as tracers for radionuclide molecular imaging of HER2- and IGF-1R-expressing tumors, paper I-IV, and for surface knock-down of EGFR, paper V. LÄS MER