Sökning: "exonuclease stability"
Visar resultat 1 - 5 av 6 avhandlingar innehållade orden exonuclease stability.
1. Conformationally Constrained Nucleosides, Nucleotides and Oligonucleotides : Design, Synthesis and Properties
Sammanfattning : This thesis is based on six original research publications describing synthesis, structure and physicochemical and biochemical analysis of chemically modified oligonucleotides (ONs) in terms of their potential diagnostic and therapeutic applications. Synthesis of two types of bicyclic conformationally constrained nucleosides, North-East locked 1',2'-azetidine and North locked 2',4'-aza-ENA, is described. LÄS MER
2. Targeting RNA by the Antisense Approach and a Close Look at RNA Cleavage Reaction
Sammanfattning : This thesis summarizes the results of studies on two aspects of nucleic acids. Chemically modified antisense oligonucleotides (AONs) have been evaluated with regards to their suitability for mRNA targeting in an antisense approach (Paper I – III). LÄS MER
3. Development of a new PNA analogue as a potential antisense drug and tool for life-science studies
Sammanfattning : The work described in this thesis focuses on applying synthetic organic chemistry methods (supported by modem synthesizers and analytical techniques) for the preparation of peptides and modified nucleic acids in order to affect certain properties in these biomolecules, which can lead to their application as drugs and/or tools for life sciences studies. The first part of the thesis reports the design and synthesis of a novel pyrrolidine-based peptide nucleic acid (PNA). LÄS MER
4. Advancements in Firefly Luciferase-Based Assays and Pyrosequencing Technology
Sammanfattning : Pyrosequencing is a new DNA sequencing method relying on thesequencing-by-synthesis principle and bioluminometric detectionof nucleotide incorporation events. The objective of thisthesis was improvement of the Pyrosequencing method byincreasing the thermal stability of firefly luciferase, and byintroducing an alternative DNA polymerase and a new nucleotideanalog. LÄS MER
5. Conformationally Constrained Nucleosides : Design, Synthesis, and Biochemical Evaluation of Modified Antisense Oligonucleotides
Sammanfattning : This thesis is concerned with synthesis, structure and biochemical analysis of chemically modified oligonucleotides with potential therapeutic applications. The three types of chemical modifications described here are: (a) A North-East locked 1',2'-azetidine nucleoside (b) A North locked 2',4'-cyanomethylene bridged nucleoside and (c) A 2',4'-aza-ENA-T nucleoside. LÄS MER