Sökning: "enzymologi Proteiner enzymology E. coli Immobilised metal ion affinity chromatography Affinity tags Green fluorescent protein Surface plasmon resonance Genetic engineering Screening Proteins Immunoassay Biotechnology Bioteknik"

Hittade 1 avhandling innehållade orden enzymologi Proteiner enzymology E. coli Immobilised metal ion affinity chromatography Affinity tags Green fluorescent protein Surface plasmon resonance Genetic engineering Screening Proteins Immunoassay Biotechnology Bioteknik.

1. 1. Metal Binding Tags - Characterisation, Use in Bioseparation and Applications of Green Fluorescent Fusion Proteins

   Författare :Florent Bernaudat; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteiner; enzymologi; enzymology; E. coli; Immobilised metal ion affinity chromatography; Affinity tags; Green fluorescent protein; Surface plasmon resonance; Genetic engineering; Screening; Proteins; Immunoassay; Biotechnology; Bioteknik;

   Sammanfattning : Most of the recombinant proteins produced nowadays are fused to affinity tags, in order to facilitate their purification through affinity chromato-graphy. Out the different tags available, poly-histidine tags are among the most commonly used and can help purification through immobilised metal ion affinity chromatography (IMAC). LÄS MER