Sökning: "enzyme purification"

Visar resultat 1 - 5 av 94 avhandlingar innehållade orden enzyme purification.

1. 1. On Catalytic Mechanisms for Rational Enzyme Design Strategies

   Författare :Charlotte Kürten; Per-Olof Syrén; Rudolf Allemann; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; catalytic mechanisms; terpene cyclase; triterpene cyclase; solvent dynamics; protein hydration; thermodynamics; quantum tunneling; polycyclization; natural compounds; ? ?-hydrolase; esterase; amidase; enzyme engineering; biocatalysis; Bioteknologi; Biotechnology;

   Sammanfattning : Enzymes enable life by promoting chemical reactions that govern the metabolism of all living organisms. As green catalysts, they have been extensively used in industry. However, to reach their full potential, engineering is often required, which can benefit from a detailed understanding of the underlying reaction mechanism. LÄS MER

3. 2. Substance P Endopeptidase : Purification and Characterizataion of Enzyme Activity and Evaluation of its Function during Stressful Condition

   Författare :Krister Karlsson; Lars Oreland; Fred Nyberg; Tsukasa Sakurada; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pharmacology; Substance P Endopeptidase; Neuropeptide; Central Nervous System; Purification; Drug Dependence; Heat Stress; Farmakologi; Pharmacological research; Farmakologisk forskning;

   Sammanfattning : The purification and biochemical characterization of the substance P (SP) hydrolyzing enzyme, substance P endopeptidase (SPE), have been carried out; with subsequent orientation in neurobiological fundamental processes involved in opioid dependence, withdrawal, and heat-stress.SPE was purified from rat spinal cord, human spinal cord and cerebrospinal fluid (CSF), rat ventral tegemental area (VTA), and rat hippocampus. LÄS MER

4. 3. Optimizing Enzyme Immobilization in Mesoporous Silica - A Spectroscopic Study of the Dynamics and Spatial Distribution of the Confined Enzymes

   Författare :Pegah Sadat Nabavi Zadeh; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; fluorescence spectroscopy; dynamics; mesoporous silica; Immunogold staining; Transmission electron microscopy; anisotropy; microviscosity; real-time monitoring; Cy5.; Förster resonance energy transfer; Enzyme immobilization; Cy3;

   Sammanfattning : Enzymes as biological catalysts are immobilized in porous materials to improve the enzyme activity and simplify their purification from the reaction media in biocatalytic applications. Mesoporous silica (MPS) particles are used as a promising support material for the immobilization of enzymes. LÄS MER

5. 4. Engineering Candida antarctica Lipase A for Enantioselective Transformations in Organic Synthesis : Design, Immobilization and Organic Solvent Screening of Smart Enzyme Libraries

   Författare :Ylva Wikmark; Jan-Erling Bäckvall; Romas Kazlauskas; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Candida antarctica Lipase A; protein engineering; enzyme libraries; enzyme immobilization; biocatalysis; organisk kemi; Organic Chemistry;

   Sammanfattning : The use of enzymes as catalysts in organic synthesis constitutes an attractive alternative to conventional chemical catalysis. Enzymes are non-toxic and biodegradable and they can operate under mild reaction conditions. LÄS MER

7. 5. Combinatorial protein engineering applied to enzyme catalysis and molecular recognition

   Författare :Malin Eklund; KTH; []
   Nyckelord :phage display; selection; enzyme; affinity domains; crystal structure; affibody; lipase; protein engineering; protein A; assembly;

   Sammanfattning : The recent development of methods for constructing andhandling large collections (libraries) of proteins, from whichvariants with desired traits can be isolated, hasrevolutionized the field of protein engineering. Key elementsof such methods are the various ways in which the genotypes(the genes) and the phenotypes (the encoded proteins) arephysically linked during the process. LÄS MER