Sökning: "enzyme intermediate"

Visar resultat 1 - 5 av 125 avhandlingar innehållade orden enzyme intermediate.

  1. 1. Intermediate State Stabilisation in Enzyme Catalysis Structural studies of two serine proteases and a photosynthetic reaction centre

    Författare :Gergely Katona; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; photosynthetic reaction centre; X-ray crystallography; structural mechanism; enzyme kinetics; serine protease; enzyme intermediate; enzyme catalysis;

    Sammanfattning : Enzymes play a key role in every organisms life. They catalyse chemical reactions and may also respond to external stimulus. The reoccurring theme of this thesis is how biologically relevant information can be gleaned from X-ray structures of the enzymes. LÄS MER

  3. 2. Specification of Dorsal and Intermediate Telencephalic Character

    Författare :Matthew Marklund; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Molecular biology; telencephalon; forebrain; dorsoventral patterning; WNT; FGF; RA; retinoid-mediated signaling; development; specification; cortex; striatal; intermediate; Molekylärbiologi; Molecular biology; Molekylärbiologi; molekylärbiologi; Molecular Biology;

    Sammanfattning : The telencephalon is the most highly evolved region of the vertebrate central nervous system (CNS). The major structures of the telencephalon - the cortex and basal ganglia – derive from the dorsally positioned pallium and the ventrally positioned subpallium, respectively. LÄS MER

  4. 3. Molecular Simulation of Enzyme Catalysis and Inhibition

    Författare :Sinisa Bjelic; Johan Åqvist; Adrian J. Mulholland; Uppsala universitet; []
    Nyckelord :Theoretical chemistry; enzyme catalysis; enzyme inhibition; computer simulations; molecular dynamics; empirical valence bond method; structure-based inhibitor design; Teoretisk kemi;

    Sammanfattning : The reaction mechanisms for the hemoglobin degrading enzymes in the Plasmodium falciparum malaria parasite, plasmepsin II (Plm II) and histo-aspartic protease (HAP), have been analyzed by molecular simulations. The reaction free energy profiles, calculated by the empirical valence bond (EVB) method in combination with molecular dynamics (MD) and free energy perturbation (FEP) simulations are in good agreement with experimental data. LÄS MER

  5. 4. Theoretical modeling of metal- and enzyme catalyzed transformations

    Författare :Jonas Nyhlén; Jan-Erling Bäckvall; Timofei Privalov; Walter Thiel; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; density functinal theory; computational chemistry; directed evolution; enzyme; mechanistic studies; catalysis; ruthenium; hydrogen transfer; racemization; artificial photosynthesis; frustrated lewis pairs; hydrogen storage; Organic chemistry; Organisk kemi; organisk kemi; Organic Chemistry;

    Sammanfattning : This thesis is focused on describing and predicting catalytic reactions. The major part of the work is based on density functional theory (DFT). In some cases where the size of the investigated system precluded the use of more accurate methods molecular dynamics was employed. LÄS MER

  7. 5. Catalysis and Site-Specific Modification of Glutathione Transferases Enabled by Rational Design

    Författare :Sofia Håkansson Hederos; Lars Baltzer; Kerstin Broo; Linköpings universitet; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Catalysis; Glutathione Transferases; Rational design; Protein design; Site-specific modification; thiolester hydrolysis; Enzyme engineering; Enzymteknik;

    Sammanfattning : This thesis describes the rational design of a novel enzyme, a thiolester hydrolase, derived from human glutathione transferase (GST) A1-1 by the introduction of a single histidine residue. The first section of the thesis describes the design and the determination of the reaction mechanism. LÄS MER