Sökning: "enzyme inactivation"

Visar resultat 1 - 5 av 80 avhandlingar innehållade orden enzyme inactivation.

1. 1. Transaminase Biocatalysis: Applications and Fundamental Studies

   Författare :Federica Ruggieri; Per Berglund; Mariarita Bertoldi; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; biocatalysis; enzymatic cascade; transaminase; amine; racemization; PLP; enzyme engineering; Chromobacterium violaceum; protein stability; enzyme stability; molecular dynamics; structural biology; enzyme technology; chiral amines; kinetic resolution; Bioteknologi; Biotechnology;

   Sammanfattning : Biocatalysis is the branch of science at the intersection between chemistry and biology and specifcally dedicated to the application of natural evolvable catalysts, i.e. enzymes, in human-designed chemical processes. Among the array of promising biocatalysts, transaminases (EC 2. LÄS MER

3. 2. Stability and inactivation mechanisms of two transaminases

   Författare :Shan Chen; Per Berglund; Patrick Adlercreutz; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Amine Transaminase; Operational Stability; Inactivation Pathway; Enzyme Unfolding; Phosphate Group Binding Cup; Bioteknologi; Biotechnology;

   Sammanfattning : In the past decades, more and more enzymes are employed as biocatalysts in industrial processes because of their advantages, such as high efficiency, substrate selectivity and stereoselectivity. Among them, amine transaminases (ATAs) are pyridoxal 5’-phosphate (PLP) dependent enzymes. LÄS MER

4. 3. Immobilization of feruloyl esterases in mesoporous silica

   Författare :Christian Thörn; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Mesoporous materials; Feruloyl esterases; Protein modeling; Biocatalysis; QCM-D; Hydroxycinnamic acids; Enzyme immobilization; Silica;

   Sammanfattning : Feruloyl esterases are a class of enzymes that can be used as biocatalysts for the modification of bioactive hydroxycinnamic acids. In industry, enzyme immobilization is often necessary to stabilize the enzymes and to allow their reuse. LÄS MER

5. 4. Endogenous and exogenous factors affecting lipoprotein lipase activity

   Författare :Mikael Larsson; Gunilla Olivecrona; Aivar Lookene; Ira Tabas; Umeå universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; LPL; APOC1; APOC2; APOC3; ANGPTL4; enzyme inactivation; lipoprotein metabolism; triglycerides; fatty acids; hypertriglyceridemia; CVD; small molecule screening; structure-activity relationship; cardiovascular disease; hjärt- och kärlforskning;

   Sammanfattning : Individuals with high levels of plasma triglycerides are at high risk to develop cardiovascular disease (CVD), currently one of the major causes of death worldwide. Recent epidemiological studies show that loss-of-function mutations in the APOC3 gene lower plasma triglyceride levels and reduce the incidence of coronary artery disease. LÄS MER

7. 5. Thiopurine S-methyltransferase - characterization of variants and ligand binding

   Författare :Annica Blissing; Lars-Göran Mårtensson; Tomas Nyman; Linköpings universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; Thiopurine S-methyltransferase TPMT ; protein stability; enzyme kinetics; ligand binding; isothermal titration calorimetry ITC ; 8-anilinonaphthalene-1-sulfonic acid ANS ;

   Sammanfattning : Thiopurine S-methyltransferase (TPMT) belongs to the Class I S-adenosylmethionine-dependent methyltransferase (SAM-MT) super family of structurally related proteins. Common to the members of this large protein family is the catalysis of methylation reactions using S-adenosylmethionine (SAM) as a methyl group donor, although SAM-MTs act on a wide range of different substrates and carry out numerous biologically important functions. LÄS MER