Sökning: "enzyme assay"
Visar resultat 6 - 10 av 323 avhandlingar innehållade orden enzyme assay.
6. On bacterial formats in protein library technology
Sammanfattning : Millions of years of evolution have resulted in an immense number of different proteins, which participate in virtually every process within cells and thus are of utmost importance for allknown forms of life. In addition, there are several examples of natural proteins which have found use in applications outside their natural environment, such as the use of enzymes infood industry and washing powders or the use of antibodies in diagnostic, bioseparation or therapeutic applications. LÄS MER
7. Haemophilia B - Diagnostic Insights, Genetic Aspects and Clinical Outcomes
Sammanfattning : Haemophilia B (HB) is a rare inherited bleeding disorder caused by the deficiency of coagulation factor IX (FIX). The major clinical issues are bleedings, often targeting the joints, and the development of neutralising antibodies, i.e. inhibitors, to the FIX replacement therapy. LÄS MER
8. Aldolases for Enzymatic Carboligation : Directed Evolution and Enzyme Structure-Function Relationship Studies
Sammanfattning : The research summarized in this thesis focuses on directed evolution and enzyme mechanism studies of two aldolases: 2-deoxyribose-5-phosphate aldolase (DERA) and fructose-6-phosphate aldolase (FSA).Aldolases are nature’s own catalysts for one of the most fundamental reactions in organic chemistry: the formation of new carbon-carbon bonds. LÄS MER
9. Dynamics of Enzyme Immobilization in Mesoporous Silica Particles
Sammanfattning : The focus of this thesis is placed on the dynamic behavior of proteins in confining environments which is studied as a scientific challenge for a deeper understanding of the immobilization mechanism and a better designing of enzyme immobilization in porous materials. Enzymes are immobilized in porous materials to improve the enzyme activity and simplify their purification from the product solution in biocatalytic applications. LÄS MER
10. CRISPRi/a for investigating yeast tolerance to inhibitors in lignocellulosic hydrolysates
Sammanfattning : Saccharomyces cerevisiae has immense potential as a cell factory in various biotechnological processes where biomass from agricultural industry residues is used as feedstock. Nonetheless, the inhibitors released during the pretreatment of the biomass makes lignocellulosic hydrolysates a challenging substrate for microorganisms. LÄS MER