Sökning: "endoribonucleases"

Hittade 4 avhandlingar innehållade ordet endoribonucleases.

1. 1. Studies on endoribonuclease RNase E and its role in RNA turnover

   Författare :Björn Sohlberg; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :Gene regulation RNA processing mRNA stability endoribonucleases RNase E AUUUA-motifs Archaea;

   Sammanfattning : RNA turnover is an important level of regulation of gene expression, by which a cell can rapidly adjust to changes in its environment. The turnover of RNA is done by an efficient but not well understood mechanism. LÄS MER

3. 2. Studies of myc gene expression and a novel endoribonuclease activity

   Författare :Anders Wennborg; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :c-myc; B-myc; heat-shock; mRNA degradation; endoribonucleases; AUUUAmotifs; RNase E.;

   Sammanfattning : The general aim of this work was to study the regulation of cellular oncogene expression, with emphasis on the c-myc gene, which is de-regulated in several human and animal tumors. The steady-state expression level of c-myc mRNA or protein was analyzed in panels of human Blymphoid cell lines with different growth and transformation properties, and the general observation was a co-variation between high c-myc expression and a more malignant phenotype of the cell lines. LÄS MER

4. 3. Expression and function of ribozymes in transgenic mice

   Författare :Michael Andäng; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :hammerhead ribozyme; hairpin ribozyme; transgenic mice; HIV-1; pseudotype virus; cleavage products; cis-cleavage;

   Sammanfattning : Hammerhead and hairpin ribozymes are short RNAs that act as sequence-specific endoribonucleases by cleaving a target RNA after hybridization to complementary sequences. By changing the specific target hybridization sequence of the ribozyme, virtually any RNA can be targeted, making ribozymes a versatile tool for gene down-regulation (knockdown). LÄS MER

5. 4. Preclinical studies of ribozyme-mediated gene therapy for HIV-1

   Författare :Catharina Maijgren Steffensson; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :AIDS; antiretroviral; cis-cleavage; gene therapy; HAART; hairpin; hammerhead; HIV-1; in vitro; in vivo; ribozyme; RNA;

   Sammanfattning : Approximately 40 million people are infected with HIV-1 and AIDS is the number one cause of death in Africa as well as, the fourth leading cause of death globally. Patients in developed countries generally receive highly active antiretroviral therapy (HAART), even though the drugs are expensive and sometimes cause side-effects and treatment failure. LÄS MER