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Visar resultat 1 - 5 av 84 avhandlingar som matchar ovanstående sökkriterier.

1. 1. Mass Spectrometry with Electrospray Ionization from an Adjustable Gap

   Författare :Patrik Ek; Åsa Emmer; Johan Roeraade; Per M. Claesson; Ulrika Nilsson; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Electrospray ionization; mass spectrometry; nanoelectrospray; ESI; MS; chip; emitter; gap; liquid bridge; nozzle; peptides; Analytical chemistry; Analytisk kemi;

   Sammanfattning : In this thesis the fabrication and analytical evaluation of two new electrospray emitters utilized for mass spectrometry analysis is presented. The emitters are based on a new concept, where the spray orifice can be varied in size. The thesis is based on two papers. All present-day nanoelectrospray emitters have fixed dimensions. LÄS MER

3. 2. New methods for sensitive analysis with nanoelectrospray ionization mass spectrometry

   Författare :Patrik Ek; Johan Roeraade; Åsa Emmer; Andries P. Bruins; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; mass spectrometry; electrospray ionization; nanoelectrospray ionization; adjustable gap; emitter; miniaturization; improved sensitivity; nanoliter picoliter sample volumes; Alzheimer’s disease; amyloid-beta Aβ ; Analytical chemistry; Analytisk kemi;

   Sammanfattning : In this thesis, new methods that address some current limitations in nanoelectrospray mass spectrometry (nESI-MS) analysis are presented. One of the major objectives is the potential gain in sensitivity that can be obtained when employing the proposed techniques. LÄS MER

4. 3. Electrospray Ionization Mass Spectrometry for Determination of Noncovalent Interactions in Drug Discovery

   Författare :Kurt Benkestock; Johan Roeraade; Renato Zenobi; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Mass spectrometry; electrospray ionization; drug discovery; noncovalent interaction; complexes; human serum albumin HSA ; fatty acid binding protein FABP ; ribonuclease; ligand screening; Analytical chemistry; Analytisk kemi;

   Sammanfattning : Noncovalent interactions are involved in many biological processes in which biomolecules bind specifically and reversibly to a partner. Often, proteins do not have a biological activity without the presence of a partner, a ligand. LÄS MER

5. 4. Electrophoretic focusing in microchannels combined with mass spectrometry : Applications on amyloid beta peptides

   Författare :Saara Mikkonen; Åsa Emmer; Johan Jacksén; Tapio Kotiaho; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; amyloid beta; computer simulation; electrophoresis; electrospray ionization; isoelectric focusing; isotachophoresis; MALDI; mass spectrometry; microchannel; microchip; nano-electrospray ionization; preconcentration; separation; Kemi; Chemistry;

   Sammanfattning : Analysis of low-abundance components in small samples remains a challenge within bioanalytical chemistry, and new techniques for sample pretreatments followed by sensitive and informative detection are required. In this thesis, procedures for preconcentration and separation of proteins and peptides in open microchannels fabricated on silicon microchips are presented. LÄS MER

7. 5. Applications of nanospray desorption electrospray ionization mass spectrometry : In situ lipid and metabolite analysis from cells to tissue

   Författare :Hilde-Marléne Bergman; Ingela Lanekoff; Jonas Bergquist; Martina Marchetti-Deschmann; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Mass spectrometry; mass spectrometry imaging MSI ; nanospray desorption electrospray ionization nano-DESI ; single-cell analysis; neurotransmitter imaging; diabetic nephropathy; pneumatic nebulization; lipidomics; metabolomics; Kemi med inriktning mot analytisk kemi; Chemistry with specialization in Analytical Chemistry;

   Sammanfattning : Ambient mass spectrometry (MS) has proved to be an important addition to the bioanalytical toolbox. These methods perform analyte sampling and ionization under atmospheric pressure, and require very little sample preparation other than the sampling process in front of the machine. LÄS MER